Localization of copper and copper transporters in the human brain

Davies, Katherine M., Hare, Dominic J., Cottam, Veronica, Chen, Nicholas, Hilgers, Leon, Halliday, Glenda, Mercer, Julian F.B. and Double, Kay L. 2013, Localization of copper and copper transporters in the human brain, Metallomics, vol. 5, no. 1, pp. 43-51, doi: 10.1039/C2MT20151H.

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Title Localization of copper and copper transporters in the human brain
Author(s) Davies, Katherine M.
Hare, Dominic J.
Cottam, Veronica
Chen, Nicholas
Hilgers, Leon
Halliday, Glenda
Mercer, Julian F.B.
Double, Kay L.
Journal name Metallomics
Volume number 5
Issue number 1
Start page 43
End page 51
Total pages 9
Publisher RSC Publications
Place of publication Cambridge, England
Publication date 2013
ISSN 1756-591X
Summary Disturbances in brain copper result in rare and severe neurological disorders and may play a role in the pathogenesis and progression of multiple neurodegenerative diseases. Our current understanding of mammalian brain copper transport is based on model systems outside the central nervous system and no data are available regarding copper transport systems in the human brain. To address this deficit, we quantified regional copper concentrations and examined the distribution and cellular localization of the copper transport proteins Copper transporter 1, Atox1, ATP7A, and ATP7B in multiple regions of the human brain using inductively coupled plasma-mass spectrometry, Western blot and immunohistochemistry. We identified significant relationships between copper transporter levels and brain copper concentrations, supporting a role for these proteins in copper transport in the human brain. Interestingly, the substantia nigra contained twice as much copper than that in other brain regions, suggesting an important role for copper in this brain region. Furthermore, ATP7A levels were significantly greater in the cerebellum, compared with other brain regions, supporting an important role for ATP7A in cerebellar neuronal health. This study provides novel data regarding copper regulation in the human brain, critical to understand the mechanisms by which brain copper levels can be altered, leading to neurological disease.
Language eng
DOI 10.1039/C2MT20151H
Field of Research 059999 Environmental Sciences not elsewhere classified
Socio Economic Objective 970105 Expanding Knowledge in the Environmental Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30055444

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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