Exercise-induced muscle glucose uptake in mice with graded, muscle-specific GLUT-4 deletion

Howlett, Kirsten F., Andrikopoulos, Sofianos, Proietto, Joseph and Hargreaves, Mark 2013, Exercise-induced muscle glucose uptake in mice with graded, muscle-specific GLUT-4 deletion, Physiological reports, vol. 1, no. 3, Article e00065, pp. 1-7, doi: 10.1002/phy2.65.

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Title Exercise-induced muscle glucose uptake in mice with graded, muscle-specific GLUT-4 deletion
Author(s) Howlett, Kirsten F.ORCID iD for Howlett, Kirsten F. orcid.org/0000-0002-8571-4867
Andrikopoulos, Sofianos
Proietto, Joseph
Hargreaves, Mark
Journal name Physiological reports
Volume number 1
Issue number 3
Season Article e00065
Start page 1
End page 7
Total pages 7
Publisher Wiley-Blackwell Publishing
Place of publication Chichester, England
Publication date 2013-08
ISSN 2051-817X
Keyword(s) exercise
muscle glucose uptake
Summary To investigate the importance of the glucose transporter GLUT-4 for muscle glucose uptake during exercise, transgenic mice with skeletal muscle GLUT-4 expression approximately 30–60% of normal (CON) and approximately 5–10% of normal (KO) were generated using the Cre/Lox system and compared with wild-type (WT) mice during approximately 40 min of treadmill running (KO: 37.7 ± 1.3 min; WT: 40 min; CON: 40 min, P = 0.18). In WT and CON animals, exercise resulted in an overall increase in muscle glucose uptake. More specifically, glucose uptake was increased in red gastrocnemius of WT mice and in the soleus and red gastrocnemius of CON mice. In contrast, the exercise-induced increase in muscle glucose uptake in all muscles was completely abolished in KO mice. Muscle glucose uptake increased during exercise in both red and white quadriceps of WT mice, while the small increases in CON mice were not statistically significant. In KO mice, there was no change at all in quadriceps muscle glucose uptake. No differences in muscle glycogen use during exercise were observed between any of the groups. However, there was a significant increase in plasma glucose levels after exercise in KO mice. The results of this study demonstrated that a reduction in skeletal muscle GLUT-4 expression to approximately 10% of normal levels completely abolished the exercise-induced increase in muscle glucose uptake.
Language eng
DOI 10.1002/phy2.65
Field of Research 111199 Nutrition and Dietetics not elsewhere classified
Socio Economic Objective 920104 Diabetes
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2013
Copyright notice ©2013, Wiley-Blackwell Publishing
Persistent URL http://hdl.handle.net/10536/DRO/DU:30057035

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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