Regulation of miRNAs in human skeletal muscle following acute endurance exercise and short-term endurance training

Russell, Aaron P., Lamon, Severine, Boon, Hanneke, Wada, Shogo, Guller, Isabelle, Brown, Erin L., Chibalin, Alexander V., Zierath, Juleen R., Snow, Rod J., Stepto, Nigel, Wadley, Glenn D. and Akimoto, Takayuki 2013, Regulation of miRNAs in human skeletal muscle following acute endurance exercise and short-term endurance training, Journal of physiology, vol. 591, no. 18, pp. 4637-4653.

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Title Regulation of miRNAs in human skeletal muscle following acute endurance exercise and short-term endurance training
Author(s) Russell, Aaron P.ORCID iD for Russell, Aaron P.
Lamon, SeverineORCID iD for Lamon, Severine
Boon, Hanneke
Wada, Shogo
Guller, Isabelle
Brown, Erin L.
Chibalin, Alexander V.
Zierath, Juleen R.
Snow, Rod J.ORCID iD for Snow, Rod J.
Stepto, Nigel
Wadley, Glenn D.ORCID iD for Wadley, Glenn D.
Akimoto, Takayuki
Journal name Journal of physiology
Volume number 591
Issue number 18
Start page 4637
End page 4653
Total pages 17
Publisher Wiley - Blackwell Publishing
Place of publication Chichester, England
Publication date 2013-09
ISSN 0022-3751
Keyword(s) microRNAs
skeletal muscle adaptation
muscle myopathies
moderate-intensity endurance cycling
Summary The identification of microRNAs (miRNAs) has established new mechanisms that control skeletal muscle adaptation to exercise. The present study investigated the mRNA regulation of components of the miRNA biogenesis pathway (Drosha, Dicer and Exportin-5), muscle enriched miRNAs, (miR-1, -133a, -133b and -206), and several miRNAs dysregulated in muscle myopathies (miR-9, -23, -29, -31 and -181). Measurements were made in muscle biopsies from nine healthy untrained males at rest, 3 h following an acute bout of moderate-intensity endurance cycling and following 10 days of endurance training. Bioinformatics analysis was used to predict potential miRNA targets. In the 3 h period following the acute exercise bout, Drosha, Dicer and Exportin-5, as well as miR-1, -133a, -133-b and -181a were all increased. In contrast miR-9, -23a, -23b and -31 were decreased. Short-term training increased miR-1 and -29b, while miR-31 remained decreased. Negative correlations were observed between miR-9 and HDAC4 protein (r=-0.71; P= 0.04), miR-31 and HDAC4 protein (r =-0.87; P= 0.026) and miR-31 and NRF1 protein (r =-0.77; P= 0.01) 3 h following exercise. miR-31 binding to the HDAC4 and NRF1 3′ untranslated region (UTR) reduced luciferase reporter activity. Exercise rapidly and transiently regulates several miRNA species in muscle. Several of these miRNAs may be involved in the regulation of skeletal muscle regeneration, gene transcription and mitochondrial biogenesis. Identifying endurance exercise-mediated stress signals regulating skeletal muscle miRNAs, as well as validating their targets and regulatory pathways post exercise, will advance our understanding of their potential role/s in human health
Language eng
Field of Research 110602 Exercise Physiology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2013, Wiley-Blackwell Publishing
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Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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