Surface-scribed transparency-based microplates

Li, Xin Ye, Cheong, Brandon Huey-Ping, Somers, Anthony, Liew, Oi Wah and Ng, Tuck Wah 2013, Surface-scribed transparency-based microplates, Langmuir, vol. 29, no. 2, pp. 849-855, doi: 10.1021/la304394s.

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Title Surface-scribed transparency-based microplates
Author(s) Li, Xin Ye
Cheong, Brandon Huey-Ping
Somers, Anthony
Liew, Oi Wah
Ng, Tuck Wah
Journal name Langmuir
Volume number 29
Issue number 2
Start page 849
End page 855
Total pages 7
Publisher American Chemical Society
Place of publication Washington, D.C.
Publication date 2013
ISSN 0743-7463
Summary Transparency sheets, which are normally associated with use on overhead projectors, offer lowered costs and high amenability for optical diagnostics in microplate instrumentation. An alternative microplate design in which circles are scribed on the surface of the transparency to create the boundaries to hold the drop in place is investigated here. The 3D profile of the scribed regions obtained optically showed strong likelihood of affecting three-phase contact line interactions. During dispensation, the contact angle (?95°) was larger than the drop advancing state (?80°) due to a period of nonadhesion, where the contact angle later reduced to the drop advancing state followed by increase in the liquid area coverage on the substrate. It was established that 50 ?L was needed to fill the well fully, and the maximum volume retainable before breaching was 190 ?L. While the tilt angle needed for displacement reduced significantly from 50 to 95 ?L, this was markedly better than nonscribed surfaces, where tilt angles always had to be kept to within 30°. It was found that there was greater ability to fill the well with smaller volumes with dispensation at the center. This was attributed to the growing contact line not meeting the scribed edge in parallel if liquid was dispensed closer to it, wherein pinning reduction in some directions permitted liquid travel along the scribed edge to undergo contact angle hysteresis. Fluorescence measurements conducted showed no performance compromise when using scribed transparency microplates over standard microplates.
Language eng
DOI 10.1021/la304394s
Field of Research 099999 Engineering not elsewhere classified
Socio Economic Objective 970109 Expanding Knowledge in Engineering
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2013, American Chemical Society
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Document type: Journal Article
Collection: Institute for Frontier Materials
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