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Genetic composition of the Ascension Island green turtle rookery based on mitochondrial DNA : implications for sampling and diversity

Formia, Angela, Broderick, Annette C., Glen, Fiona, Godley, Brendan J., Hays, Graeme C. and Bruford, Michael W. 2007, Genetic composition of the Ascension Island green turtle rookery based on mitochondrial DNA : implications for sampling and diversity, Endangered species research, vol. 3, no. 2, pp. 145-158, doi: 10.3354/esr003145.

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Title Genetic composition of the Ascension Island green turtle rookery based on mitochondrial DNA : implications for sampling and diversity
Author(s) Formia, Angela
Broderick, Annette C.
Glen, Fiona
Godley, Brendan J.
Hays, Graeme C.ORCID iD for Hays, Graeme C. orcid.org/0000-0002-3314-8189
Bruford, Michael W.
Journal name Endangered species research
Volume number 3
Issue number 2
Start page 145
End page 158
Total pages 14
Publisher Inter-Research
Place of publication Oldendorf, Germany
Publication date 2007-10
ISSN 1863-5407
1613-4796
Keyword(s) Chelonia mydas
control region
sequences
Haplotypes
analysis of variance
Rarefaction analysis
Summary In species of conservation concern it is often difficult to be certain that population diversity and structure have been adequately characterised by genetic sampling. Since practical and financial constraints tend to be associated with increasing sample sizes in many conservation genetic studies, it is important to consider the potential for sampling error and bias due to inadequate samples or spatio-temporal structure within populations. We analysed sequence data from the mitochondrial DNA control region in a large sample (n = 245) of green sea turtles Chelonia mydas collected at the globally important rookery of Ascension Island, South Atlantic. We examined genetic diversity and structure among 10 sampling sites, 4 beach clusters and 4 nesting seasons, and evaluated the genetic composition of Ascension against other Atlantic nesting populations, including the well-studied rookery at Tortuguero (Costa Rica). Finally, we used rarefaction and GENESAMP analyses to assess the ability of different sample sizes to provide acceptable genetic representations of a population, using Ascension and Tortuguero as models. On Ascension, we found 13 haplotypes, of which only 3 had been previously observed in the rookery, and 5 previously undescribed. We detected no differentiation among beach clusters or sampling seasons, and only weak differentiation among the 3 primary nesting sites. The increased sample size for Ascension provided higher resolution and statistical power in describing genetic structure among all other known Atlantic rookeries. Our extrapolations showed that a maximum of 18 and 6 haplotypes are expected to occur in Ascension and Tortuguero, respectively, and that current sample sizes are sufficient to describe most of the variation. We recommend using rarefaction and GENESAMP analyses on a rookery-by-rookery basis to evaluate whether a sample set adequately describes mitochondrial DNA diversity, thus strengthening subsequent phylogeographic and mixed stock analyses, and management recommendations for conservation.
Language eng
DOI 10.3354/esr003145
Field of Research 069999 Biological Sciences not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2007, Inter-Research
Persistent URL http://hdl.handle.net/10536/DRO/DU:30058353

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.