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Measurement of immunoglobulin A in saliva by particle-enhanced nephelometric immunoassay: sample collection, limits of quantitation, precision, stability and reference range

Booth, Christine K., Dwyer, Dan B., Pacque, Paul F. and Ball, Madeleine J. 2009, Measurement of immunoglobulin A in saliva by particle-enhanced nephelometric immunoassay: sample collection, limits of quantitation, precision, stability and reference range, Annals of clinical biochemistry, vol. 46, no. 5, pp. 401-406, doi: 10.1258/acb.2009.008248.

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Title Measurement of immunoglobulin A in saliva by particle-enhanced nephelometric immunoassay: sample collection, limits of quantitation, precision, stability and reference range
Author(s) Booth, Christine K.
Dwyer, Dan B.
Pacque, Paul F.
Ball, Madeleine J.
Journal name Annals of clinical biochemistry
Volume number 46
Issue number 5
Start page 401
End page 406
Total pages 6
Publisher Sage
Place of publication London, England
Publication date 2009-09
ISSN 0004-5632
Summary Background: Total immunoglobulin A in saliva (s-IgA) is normally assayed using an enzyme-linked immunosorbent assay. We have investigated methodological issues relating to the use of particle-enhanced nephelometric immunoassay (PENIA)
to measure s-IgA in whole unstimulated saliva and determine its reference range.

Methods: Whole unstimulated resting saliva was collected to determine sample stability (temperature, time, effect of a protease inhibitor), limit of quantitation (LOQ), assay precision and analytical variation. The reference range for 134 healthy adults was determined.

Results: Linearity was excellent (4–10.3 mg L21, P, 0.001; R2 ¼ 0.997) and without significant bias (mean of 20.7%). The lowest intra- and inter-analytical coefficients of variation were 1.8% and 7.5% and LOQ was 1.4 mg L21. The concentration of s-IgA is stable at room temperature for up to 6 h, at 48C for 48 h, at 248C for two weeks and at 2808C for up to 1.3 yr. There is no evidence that a protease inhibitor increases the stability or that repeated freeze–thawing cycles degrade sample quality. The reference ranges for s-IgA concentration, s-IgA secretion, s-IgA:albumin and s-IgA:osmolality were 15.9–414.5 mg L21, 7.2–234.9 mg min21, 0.4–19 and 0.6–8.9, respectively.

Conclusion:
Automated PENIA assay of s-IgA is precise and accurate. High stability of collected saliva samples and the ease and speed of the assay make this an ideal method for use in athletic and military training situations. The convenience of measuring albumin and IgA on the same analytical platform adds to the practicability of the test.
Language eng
DOI 10.1258/acb.2009.008248
Field of Research 110602 Exercise Physiology
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2009, Sage Publications
Persistent URL http://hdl.handle.net/10536/DRO/DU:30058715

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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Created: Mon, 02 Dec 2013, 13:41:19 EST by Dan Dwyer

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