Metabolic profile analysis of zebrafish embryos

Gibert, Yann, McGee, Sean L. and Ward, Alister C. 2013, Metabolic profile analysis of zebrafish embryos, Journal of visualized experiments, vol. 71, Article e4300, pp. 1-5.

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Title Metabolic profile analysis of zebrafish embryos
Author(s) Gibert, Yann
McGee, Sean L.
Ward, Alister C.
Journal name Journal of visualized experiments
Volume number 71
Season Article e4300
Start page 1
End page 5
Total pages 5
Publisher Journal of Visualized Experiments
Place of publication Cambridge, Massachusetts
Publication date 2013
ISSN 1940-087X
Summary A growing goal in the field of metabolism is to determine the impact of genetics on different aspects of mitochondrial function. Understanding these relationships will help to understand the underlying etiology for a range of diseases linked with mitochondrial dysfunction, such as diabetes and obesity. Recent advances in instrumentation, has enabled the monitoring of distinct parameters of mitochondrial function in cell lines or tissue explants. Here we present a method for a rapid and sensitive analysis of mitochondrial function parameters in vivo during zebrafish embryonic development using the Seahorse bioscience XF 24 extracellular flux analyser. This protocol utilizes the Islet Capture microplates where a single embryo is placed in each well, allowing measurement of bioenergetics, including: (i) basal respiration; (ii) basal mitochondrial respiration (iii) mitochondrial respiration due to ATP turnover; (iv) mitochondrial uncoupled respiration or proton leak and (iv) maximum respiration. Using this approach embryonic zebrafish respiration parameters can be compared between wild type and genetically altered embryos (mutant, gene over-expression or gene knockdown) or those manipulated pharmacologically. It is anticipated that dissemination of this protocol will provide researchers with new tools to analyse the genetic basis of metabolic disorders in vivo in this relevant vertebrate animal model.
Language eng
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 920104 Diabetes
HERDC Research category C1 Refereed article in a scholarly journal
Restricted until 2015
Copyright notice ©2013, JoVE
Persistent URL http://hdl.handle.net/10536/DRO/DU:30058924

Document type: Journal Article
Collection: School of Medicine
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