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Inhibition of A/Human/Hubei/3/2005 (H3N2) influenza virus infection by silver nanoparticles in vitro and in vivo

Xiang, Dongxi, Yang, Zheng, Duan, Wei, Li, Xiujing, Yin, Jianjian, Shigdar, Sarah, O'Connor, Michael Liam, Marappan, Manju, Zhao, Xiaojuan, Miao, Yingqiu, Xiang, Bin and Zheng, Conglong 2013, Inhibition of A/Human/Hubei/3/2005 (H3N2) influenza virus infection by silver nanoparticles in vitro and in vivo, International Journal of Nanomedicine, vol. 8, no. 1, pp. 4103-4114.

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Title Inhibition of A/Human/Hubei/3/2005 (H3N2) influenza virus infection by silver nanoparticles in vitro and in vivo
Author(s) Xiang, Dongxi
Yang, Zheng
Duan, Wei
Li, Xiujing
Yin, Jianjian
Shigdar, Sarah
O'Connor, Michael Liam
Marappan, Manju
Zhao, Xiaojuan
Miao, Yingqiu
Xiang, Bin
Zheng, Conglong
Journal name International Journal of Nanomedicine
Volume number 8
Issue number 1
Start page 4103
End page 4114
Total pages 12
Publisher Dove Medical Press
Place of publication Macclesfield, UK
Publication date 2013
ISSN 1178-2013
Keyword(s) Silver nanoparticles
Antiviral activity
H3N2
Influenza virus
Summary Abstract
Silver nanoparticles (AgNPs) have attracted much attention as antimicrobial agents and have demonstrated efficient inhibitory activity against various viruses, including human immunodeficiency virus, hepatitis B virus, and Tacaribe virus. In this study, we investigated if AgNPs could have antiviral and preventive effects in A/Human/Hubei/3/2005 (H3N2) influenza virus infection. Madin-Darby canine kidney cells infected with AgNP-treated H3N2 influenza virus showed better viability (P,0.05 versus influenza virus control) and no obvious cytopathic effects compared with an influenza virus control group and a group treated with the solvent used for preparation of the AgNPs. Hemagglutination assay indicated that AgNPs could significantly inhibit growth of the influenza virus in Madin-Darby canine kidney cells (P,0.01 versus the influenza virus control). AgNPs significantly reduced cell apoptosis induced by H3N2 influenza virus at three different treatment pathways (P,0.05 versus influenza virus control). H3N2 influenza viruses treated with AgNPs were analyzed by transmission electron microscopy and found to interact with each other, resulting in destruction of morphologic viral structures in a time-dependent manner in a time range of 30 minutes to 2 hours. In addition, intranasal AgNP administration in mice significantly enhanced survival after infection with the H3N2 influenza virus. Mice treated with AgNPs showed lower lung viral titer levels and minor pathologic lesions in lung tissue, and had a marked survival benefit during secondary intranasal passage in vivo. These results provide evidence that AgNPs have beneficial effects in preventing H3N2 influenza virus infection both in vitro and in vivo, and demonstrate that AgNPs can be used as potential therapeutics for inhibiting outbreaks of influenza.
Language eng
Field of Research 100703 Nanobiotechnology
Socio Economic Objective 920109 Infectious Diseases
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2013, Dove Medical Press
Persistent URL http://hdl.handle.net/10536/DRO/DU:30059383

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Collection
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.