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Intracellular dynamics of HIV infection

Petravic, Janka, Ellenberg, Paula, Chan, Ming-Liang, Paukovics, Geza, Smyth, Redmond P., Mak, Johnson and Miles, Davenport P. 2014, Intracellular dynamics of HIV infection, Journal of virology, vol. 88, no. 2, pp. 1113-1124, doi: 10.1128/JVI.02038-13.

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Title Intracellular dynamics of HIV infection
Author(s) Petravic, Janka
Ellenberg, Paula
Chan, Ming-Liang
Paukovics, Geza
Smyth, Redmond P.
Mak, JohnsonORCID iD for Mak, Johnson orcid.org/0000-0002-5229-5707
Miles, Davenport P.
Journal name Journal of virology
Volume number 88
Issue number 2
Start page 1113
End page 1124
Total pages 12
Publisher American Society for Microbiology
Place of publication Washington DC
Publication date 2014-01
ISSN 0022-538X
1098-5514
Summary Early studies of HIV infection dynamics suggested that virus-producing HIV-infected cells had an average half-life of approximately 1 day. However, whether this average behavior is reflective of the dynamics of individual infected cells is unclear. Here, we use HIV-enhanced green fluorescent protein (EGFP) constructs and flow cytometry sorting to explore the dynamics of cell infection, viral protein production, and cell death in vitro. By following the numbers of productively infected cells expressing EGFP over time, we show that infected cell death slows down over time. Although infected cell death in vivo could be very different, our results suggest that the constant decay of cell numbers observed in vivo during antiretroviral treatment could reflect a balance of cell death and delayed viral protein production. We observe no correlation between viral protein production and death rate of productively infected cells, showing that viral protein production is not likely to be the sole determinant of the death of HIV-infected cells. Finally, we show that all observed features can be reproduced by a simple model in which infected cells have broad distributions of productive life spans, times to start viral protein production, and viral protein production rates. This broad spectrum of the level and timing of viral protein production provides new insights into the behavior and characteristics of HIV-infected cells.
Language eng
DOI 10.1128/JVI.02038-13
Field of Research 110804 Medical Virology
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2014, American Society for Microbiology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30059491

Document type: Journal Article
Collections: School of Medicine
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Created: Tue, 14 Jan 2014, 13:21:45 EST by Jane Moschetti

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.