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Peptide-fluorescent bacteria complex as luminescent reagents for cancer diagnosis

Dong, Bing, Wang, Anxin, Yuan, Lihua, Chen, Lisha, Pu, Kefeng, Duan, Wei, Yan, Xiyun and Zhu, Yimin 2013, Peptide-fluorescent bacteria complex as luminescent reagents for cancer diagnosis, PLOS One, vol. 8, no. 1, Article e54467.

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Title Peptide-fluorescent bacteria complex as luminescent reagents for cancer diagnosis
Author(s) Dong, Bing
Wang, Anxin
Yuan, Lihua
Chen, Lisha
Pu, Kefeng
Duan, Wei
Yan, Xiyun
Zhu, Yimin
Journal name PLOS One
Volume number 8
Issue number 1
Season Article e54467
Total pages 9
Publisher Public Library of Science
Place of publication San Francisco, California
Publication date 2013-01
ISSN 1932-6203
Keyword(s) immunohistochemistry methods
genres of cancer
peptide-fluorescent bacteria
human pathological samples
binding peptides
diagnostic tool
Summary Currently in clinic, people use hematoxylin and eosin stain (H&E stain) and immunohistochemistry methods to identify the generation and genre of cancers for human pathological samples. Since these methods are inaccurate and time consuming, developing a rapid and accurate method to detect cancer is urgently demanded. In our study, binding peptides for lung cancer cell line A549 were identified using bacteria surface display method. With those binding peptides for A549 cells on the surface, the fluorescent bacteria (Escherichia coli with stably expressed green fluorescent protein) were served as specific detecting reagents for the diagnosis of cancers. The binding activity of peptide-fluorescent bacteria complex was confirmed by detached cancer cells, attached cancer cells and mice tumor xenograft samples. A unique fixation method was developed for peptide-bacteria complex in order to make this complex more feasible for the clinic use. This peptide-fluorescent bacteria complex has great potential to become a new diagnostic tool for clinical application.
Language eng
Field of Research 111504 Pharmaceutical Sciences
Socio Economic Objective 920102 Cancer and Related Disorders
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2013, Public Library of Science
Persistent URL http://hdl.handle.net/10536/DRO/DU:30059551

Document type: Journal Article
Collections: School of Medicine
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.