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Addition of magnesium chloride to enhance mono-dispersity of a coiled-coil recombinant mouse macrophage protein

Pahuja, Parveen, Srinivasan, Alagiri and Puri, Munish 2014, Addition of magnesium chloride to enhance mono-dispersity of a coiled-coil recombinant mouse macrophage protein, Molecular and cellular biochemistry, vol. 389, no. 1-2, pp. 133-139, doi: 10.1007/s11010-013-1934-x.

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Title Addition of magnesium chloride to enhance mono-dispersity of a coiled-coil recombinant mouse macrophage protein
Author(s) Pahuja, Parveen
Srinivasan, Alagiri
Puri, MunishORCID iD for Puri, Munish orcid.org/0000-0003-2469-3326
Journal name Molecular and cellular biochemistry
Volume number 389
Issue number 1-2
Start page 133
End page 139
Total pages 7
Publisher Springer
Place of publication Berlin, Germany
Publication date 2014
ISSN 0300-8177
1573-4919
Keyword(s) Crystallisation
Dispersity
Dynamic light scattering
Gel filtration chromatography
Summary X-ray crystallography for the determination of three-dimensional structures of protein macromolecules represents an important tool in function assignment of uncharacterized proteins. However, crystallisation is often difficult to achieve. A protein sample fully characterized in terms of dispersity may increase the likelihood of successful crystallisation by improving the predictability of the crystallisation process. To maximize the probability of crystallisation of a novel mouse macrophage protein (rMMP), target molecule was characterized and refined to improve monodispersity. Addition of MgCl2 at low concentrations resolves the rMMP into a monodisperse solution, and finally successful crystallization of rMMP was achieved. The effect of MgCl2 was studied using gel filtration chromatography and dynamic light scattering.
Language eng
DOI 10.1007/s11010-013-1934-x
Field of Research 059999 Environmental Sciences not elsewhere classified
Socio Economic Objective 970105 Expanding Knowledge in the Environmental Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2014, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30060836

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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