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Determination of neurotransmitters and their metabolites using one- and two-dimensional liquid chromatography with acidic potassium permanganate chemiluminescence detection

Holland, Brendan J., Conlan, Xavier A., Stevenson, Paul G., Tye, Susannah, Reker, Ashlie, Barnett, Neil W., Adcock, Jacqui L. and Francis, Paul S. 2014, Determination of neurotransmitters and their metabolites using one- and two-dimensional liquid chromatography with acidic potassium permanganate chemiluminescence detection, Analytical and bioanalytical chemistry, vol. 406, no. 23, pp. 5669-5676, doi: 10.1007/s00216-013-7514-9.

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Title Determination of neurotransmitters and their metabolites using one- and two-dimensional liquid chromatography with acidic potassium permanganate chemiluminescence detection
Author(s) Holland, Brendan J.
Conlan, Xavier A.ORCID iD for Conlan, Xavier A. orcid.org/0000-0003-0829-0551
Stevenson, Paul G.ORCID iD for Stevenson, Paul G. orcid.org/0000-0001-6780-6859
Tye, Susannah
Reker, Ashlie
Barnett, Neil W.
Adcock, Jacqui L.ORCID iD for Adcock, Jacqui L. orcid.org/0000-0002-4446-518X
Francis, Paul S.ORCID iD for Francis, Paul S. orcid.org/0000-0003-4165-6922
Journal name Analytical and bioanalytical chemistry
Volume number 406
Issue number 23
Start page 5669
End page 5676
Total pages 8
Publisher Springer
Place of publication Berlin, Germany
Publication date 2014
ISSN 1618-2642
1618-2650
Keyword(s) Brain
Chemiluminescence
Neurotransmitter metabolites
Neurotransmitters
Two-dimensional high-performance liquid chromatography
Urine
Summary High-performance liquid chromatography with chemiluminescence detection based on the reaction with acidic potassium permanganate and formaldehyde was explored for the determination of neurotransmitters and their metabolites. The neurotransmitters norepinephrine and dopamine were quantified in the left and right hemispheres of rat hippocampus, nucleus accumbens and prefrontal cortex, and the metabolites vanillylmandelic acid, 3,4-dihydrophenylacetic acid, 5-hydroxyindole-3-acetic acid and homovanillic acid were identified in human urine. Under optimised chemiluminescence reagent conditions, the limits of detection for these analytes ranged from 2.5 × 10−8 to 2.5 × 10−7 M. For the determination of neurotransmitter metabolites in urine, a two-dimensional high-performance liquid chromatography (2D-HPLC) separation operated in heart-cutting mode was developed to overcome the peak capacity limitations of the one-dimensional separation. This approach provided the greater separation power of 2D-HPLC with analysis times comparable to conventional one-dimensional separations.
Language eng
DOI 10.1007/s00216-013-7514-9
Field of Research 030108 Separation Science
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2014, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30060890

Document type: Journal Article
Collections: School of Psychology
School of Life and Environmental Sciences
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Created: Fri, 21 Feb 2014, 08:35:09 EST

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