Engineering a multimodal nerve conduit for repair of injured peripheral nerve

Quigley, A F, Bulluss, K J, Kyratzis, I L B, Gilmore, K, Mysore, T, Schirmer, K S U, Kennedy, E L, O'Shea, M, Truong, Y B, Edwards, S L, Peeters, G, Herwig, P, Razal, J M, Campbell, T E, Lowes, K N, Higgins, M J, Moulton, S E, Murphy, M A, Cook, M J, Clark, G M, Wallace, G G and Kapsa, R M I 2013, Engineering a multimodal nerve conduit for repair of injured peripheral nerve, Journal of Neural Engineering, vol. 10, no. 1, pp. 1-17, doi: 10.1088/1741-2560/10/1/016008.

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Title Engineering a multimodal nerve conduit for repair of injured peripheral nerve
Author(s) Quigley, A F
Bulluss, K J
Kyratzis, I L B
Gilmore, K
Mysore, T
Schirmer, K S U
Kennedy, E L
O'Shea, M
Truong, Y B
Edwards, S L
Peeters, G
Herwig, P
Razal, J MORCID iD for Razal, J M
Campbell, T E
Lowes, K N
Higgins, M J
Moulton, S E
Murphy, M A
Cook, M J
Clark, G M
Wallace, G G
Kapsa, R M I
Journal name Journal of Neural Engineering
Volume number 10
Issue number 1
Start page 1
End page 17
Total pages 17
Publisher Institute of Physics Publishing
Place of publication Bristol, England
Publication date 2013
ISSN 1741-2560
Keyword(s) Engineering
Peripheral nerve
Multimodal nerve conduit
Summary Injury to nerve tissue in the peripheral nervous system (PNS) results in long-term impairment of limb function, dysaesthesia and pain, often with associated psychological effects. Whilst minor injuries can be left to regenerate without intervention and short gaps up to 2 cm can be sutured, larger or more severe injuries commonly require autogenous nerve grafts harvested from elsewhere in the body (usually sensory nerves). Functional recovery is often suboptimal and associated with loss of sensation from the tissue innervated by the harvested nerve. The challenges that persist with nerve repair have resulted in development of nerve guides or conduits from non-neural biological tissues and various polymers to improve the prognosis for the repair of damaged nerves in the PNS. This study describes the design and fabrication of a multimodal controlled pore size nerve regeneration conduit using polylactic acid (PLA) and (PLA):poly(lactic-co-glycolic) acid (PLGA) fibers within a neurotrophin-enriched alginate hydrogel. The nerve repair conduit design consists of two types of PLGA fibers selected specifically for promotion of axonal outgrowth and Schwann cell growth (75:25 for axons; 85:15 for Schwann cells). These aligned fibers are contained within the lumen of a knitted PLA sheath coated with electrospun PLA nanofibers to control pore size. The PLGA guidance fibers within the nerve repair conduit lumen are supported within an alginate hydrogel impregnated with neurotrophic factors (NT-3 or BDNF with LIF, SMDF and MGF-1) to provide neuroprotection, stimulation of axonal growth and Schwann cell migration. The conduit was used to promote repair of transected sciatic nerve in rats over a period of 4 weeks. Over this period, it was observed that over-grooming and self-mutilation (autotomy) of the limb implanted with the conduit was significantly reduced in rats implanted with the full-configuration conduit compared to rats implanted with conduits containing only an alginate hydrogel. This indicates return of some feeling to the limb via the fully-configured conduit. Immunohistochemical analysis of the implanted conduits removed from the rats after the four-week implantation period confirmed the presence of myelinated axons within the conduit and distal to the site of implantation, further supporting that the conduit promoted nerve repair over this period of time. This study describes the design considerations and fabrication of a novel multicomponent, multimodal bio-engineered synthetic conduit for peripheral nerve repair.
Language eng
DOI 10.1088/1741-2560/10/1/016008
Field of Research 109999 Technology not elsewhere classified
Socio Economic Objective 970110 Expanding Knowledge in Technology
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2013, Institute of Physics Publishing
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