Growth of rotaviruses in continuous human and monkey cell lines that vary in their expression of integrins

Londrigan, Sarah L, Hewish, Marilyn J, Thomson, Melanie J, Sanders, Georgina M, Mustafa, Huseyin and Coulson, Barbara S 2000, Growth of rotaviruses in continuous human and monkey cell lines that vary in their expression of integrins, Journal of General Virology, vol. 81, no. 9, pp. 2203-2213.

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Title Growth of rotaviruses in continuous human and monkey cell lines that vary in their expression of integrins
Author(s) Londrigan, Sarah L
Hewish, Marilyn J
Thomson, Melanie J
Sanders, Georgina M
Mustafa, Huseyin
Coulson, Barbara S
Journal name Journal of General Virology
Volume number 81
Issue number 9
Start page 2203
End page 2213
Total pages 11
Publisher Society for General Microbiology
Place of publication London, UK
Publication date 2000
ISSN 0022-1317
1465-2099
Keyword(s) Rotaviruses
Human cell lines
Monkey cell lines
Summary Rotavirus replication occurs in vivo in intestinal epithelial cells. Cell lines fully permissive to rotavirus include kidney epithelial (MA104), colonic (Caco-2) and hepatic (HepG2) types. Previously, it has been shown that cellular integrins α2β1, α4β1 and αXβ2 are involved in rotavirus cell entry. As receptor usage is a major determinant of virus tropism, the levels of cell surface expression of these integrins have now been investigated by flow cytometry on cell lines of human (Caco-2, HepG2, RD, K562) and monkey (MA104, COS-7) origin in relation to cellular susceptibility to infection with monkey and human rotaviruses. Cells supporting any replication of human rotaviruses (RD, HepG2, Caco-2, COS-7 and MA104) expressed α2β1 and (when tested) αXβ2, whereas the non-permissive K562 cells did not express α2β1, α4β1 or αXβ2. Only RD cells expressed α4β1. Although SA11 grew to higher titres in RD, HepG2, Caco-2, COS-7 and MA104 cells, this virus still replicated at a low level in K562 cells. In all cell lines tested, SA11 replicated to higher titres than did human strains, consistent with the ability of SA11 to use sialic acids as alternative receptors. Levels of cell surface α2 integrin correlated with levels of rotavirus growth. The α2 integrin relative linear median fluorescence intensity on K562, RD, COS-7, MA104 and Caco-2 cells correlated linearly with the titre of SA11 produced in these cells at 20 h after infection at a multiplicity of 0·1, and the data best fitted a sigmoidal dose–response curve (r2=1·00, P=0·005). Thus, growth of rotaviruses in these cell lines correlates with their surface expression of α2β1 integrin and is consistent with their expression of αXβ2 and α4β1 integrins.
Language eng
Field of Research 110309 Infectious Diseases
110303 Clinical Microbiology
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30061396

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
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