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Gastric Helicobacter infection induces iron deficiency in the INS-GAS mouse

Thomson, Melanie J, Pritchard, D Mark, Boxall, Sally A, Abuderman, Abdul A, Williams, Jonathan M, Varro, Andrea and Crabtree, Jean E 2012, Gastric Helicobacter infection induces iron deficiency in the INS-GAS mouse, PLoS one, vol. 7, no. 11, pp. 1-9, doi: 10.1371/journal.pone.0050194.

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Title Gastric Helicobacter infection induces iron deficiency in the INS-GAS mouse
Formatted title Gastric Helicobacter infection induces iron deficiency in the INS-GAS mouse
Author(s) Thomson, Melanie J
Pritchard, D Mark
Boxall, Sally A
Abuderman, Abdul A
Williams, Jonathan M
Varro, Andrea
Crabtree, Jean E
Journal name PLoS one
Volume number 7
Issue number 11
Article ID e50194
Start page 1
End page 9
Total pages 9
Publisher Public Library of Science
Place of publication San Francisco, Ca.
Publication date 2012
ISSN 1932-6203
Keyword(s) Gastric Helicobacter infection
Iron deficiency
INS-GAS Mouse
Summary There is increasing evidence from clinical and population studies for a role of H. pylori infection in the aetiology of iron deficiency. Rodent models of Helicobacter infection are helpful for investigating any causal links and mechanisms of iron deficiency in the host. The aim of this study was to investigate the effects of gastric Helicobacter infection on iron deficiency and host iron metabolism/transport gene expression in hypergastrinemic INS-GAS mice. INS-GAS mice were infected with Helicobacter felis for 3, 6 and 9 months. At post mortem, blood was taken for assessment of iron status and gastric mucosa for pathology, immunohistology and analysis of gene expression. Chronic Helicobacter infection of INS- GAS mice resulted in decreased serum iron, transferrin saturation and hypoferritinemia and increased Total iron binding capacity (TIBC). Decreased serum iron concentrations were associated with a concomitant reduction in the number of parietal cells, strengthening the association between hypochlorhydria and gastric Helicobacter-induced iron deficiency. Infection with H. felis for nine months was associated with decreased gastric expression of iron metabolism regulators hepcidin, Bmp4 and Bmp6 but increased expression of Ferroportin 1, the iron efflux protein, iron absorption genes such as Divalent metal transporter 1, Transferrin receptor 1 and also Lcn2 a siderophore-binding protein. The INS-GAS mouse is therefore a useful model for studying Helicobacter-induced iron deficiency. Furthermore, the marked changes in expression of gastric iron transporters following Helicobacter infection may be relevant to the more rapid development of carcinogenesis in the Helicobacter infected INS-GAS model.
Language eng
DOI 10.1371/journal.pone.0050194
Field of Research 110309 Infectious Diseases
110303 Clinical Microbiology
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2012, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30061407

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Collection
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.