Transfection of MDA-MB-231 human breast carcinoma cells with bone sialoprotein (BSP) stimulates migration and invasion in vitro and growth of primary and secondary tumors in nude mice

Sharp, Julie A, Waltham, Mark, Williams, Elizabeth D, Henderson, Michael A and Thompson, Erik W 2004, Transfection of MDA-MB-231 human breast carcinoma cells with bone sialoprotein (BSP) stimulates migration and invasion in vitro and growth of primary and secondary tumors in nude mice, Clinical and Experimental Metastasis, vol. 21, no. 1, pp. 19-29.

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Title Transfection of MDA-MB-231 human breast carcinoma cells with bone sialoprotein (BSP) stimulates migration and invasion in vitro and growth of primary and secondary tumors in nude mice
Author(s) Sharp, Julie A
Waltham, Mark
Williams, Elizabeth D
Henderson, Michael A
Thompson, Erik W
Journal name Clinical and Experimental Metastasis
Volume number 21
Issue number 1
Start page 19
End page 29
Total pages 11
Publisher Kluwer Academic Publishers
Place of publication Dordrecht, Netherlands
Publication date 2004
ISSN 0262-0898
Keyword(s) Bone sialoprotein
Breast cancer
Metastasis
in vitro wound healing
Migration
Summary We have investigated the role of bone sialoprotein (BSP), a secreted glycoprotein normally found in bone, in breast cancer progression. To explore functions for BSP in human breast cancer invasion and metastasis, the full-length BSP cDNA was transfected into the MDA-MB-231-BAG human breast cancer cell line under the control of the CMV promoter. Clones expressing BSP and vector control clones were isolated. BSP producing clones showed increased monolayer wound healing, a faster rate of stellate outgrowth in Matrigel and increased rate of invasion into a collagen matrix when compared to control clones. Clones were also examined in models of breast cancer growth and metastasis in vivo. BSP transfected clones showed an increased rate of primary tumor growth following mammary fat pad injection of nude mice. BSP transfected clones and vector control clones metastasized to soft organs and bone at a similar rate after intra-cardiac injection as determined by real-time PCR and X-ray analysis. Although these organs were targets for both BSP transfected and non-transfected cells, the size of the metastatic lesion was shown to be significantly larger for BSP expressing clones. This was determined by real-time PCR analysis for soft organs and by X-ray analysis of bone lesions. For bone this was confirmed by intra-tibial injections of cells in nude mice. We conclude that BSP acts to drive primary and secondary tumor growth of breast cancers in vivo.
Language eng
Field of Research 111201 Cancer Cell Biology
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2004, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30061489

Document type: Journal Article
Collection: School of Medicine
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