A surface-stress-based microcantilever aptasensor

Lim, Yang Choon, Kouzani, Abbas Z., Duan, Wei, Dai, Xiujuan J., Kaynak, Akif and Mair, Douglas 2014, A surface-stress-based microcantilever aptasensor, IEEE transactions on biomedical circuits and systems, vol. 8, no. 1, pp. 15-24.

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Title A surface-stress-based microcantilever aptasensor
Author(s) Lim, Yang Choon
Kouzani, Abbas Z.
Duan, Wei
Dai, Xiujuan J.
Kaynak, Akif
Mair, Douglas
Journal name IEEE transactions on biomedical circuits and systems
Volume number 8
Issue number 1
Start page 15
End page 24
Total pages 10
Publisher IEEE
Place of publication Piscataway, NJ
Publication date 2014
ISSN 1932-4545
Keyword(s) Aptasensor
SU-8
Label-free
Microcantilever
Optical
Summary Biosensors based on microcantilevers convert biological recognition events into measurable mechanical displacements. They offer advantages such as small size, low sample volume, label-free detection, ease of integration, high-throughput analysis, and low development cost. The design and development of a microcantilever-based aptasensor employing SU-8 polymer as the fabrication material is presented in this paper. Aptamers are employed as bioreceptor elements because they exhibit superior specificity compared to antibodies due to their small size and physicochemical stability. To immobilise thrombin DNA aptamer on the bare SU-8 surface of the aptasensor, a combined plasma mode treatment method is implemented which modifies the surface of the aptasensor. Label-free detection of thrombin molecules using the fabricated aptasensor is successfully demonstrated. The measured deflection is one order of magnitude higher than that of a silicon nitride microcantilever biosensor. The developed aptasensor also demonstrates high specificity.
Language eng
Field of Research 099999 Engineering not elsewhere classified
Socio Economic Objective 970109 Expanding Knowledge in Engineering
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2014, IEEE
Persistent URL http://hdl.handle.net/10536/DRO/DU:30061683

Document type: Journal Article
Collections: School of Medicine
School of Engineering
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