You are not logged in.
Openly accessible

Purity and enrichment of laser-microdissected midbrain dopamine neurons

Brown, Amanda L., Day, Trevor A., Dayas, Christopher V. and Smith, Doug W. 2013, Purity and enrichment of laser-microdissected midbrain dopamine neurons, Biomed research international, vol. 2013, Article ID : 747938, pp. 1-8, doi: 10.1155/2013/747938.

Attached Files
Name Description MIMEType Size Downloads
day-purityandenrichment-2013.pdf Published version application/pdf 2.64MB 35

Title Purity and enrichment of laser-microdissected midbrain dopamine neurons
Author(s) Brown, Amanda L.
Day, Trevor A.
Dayas, Christopher V.
Smith, Doug W.
Journal name Biomed research international
Volume number 2013
Season Article ID : 747938
Start page 1
End page 8
Total pages 8
Publisher Hindawi Publishing Corporation
Place of publication New York, N. Y.
Publication date 2013
ISSN 2314-6141
2314-6133
Keyword(s) enrichment
purity
laser microdissection
midbrain dopamine neurons
gene expression profiles
Summary The ability to microdissect individual cells from the nervous system has enormous potential, as it can allow for the study of gene expression in phenotypically identified cells. However, if the resultant gene expression profiles are to be accurately ascribed, it is necessary to determine the extent of contamination by nontarget cells in the microdissected sample. Here, we show that midbrain dopamine neurons can be laser-microdissected to a high degree of enrichment and purity. The average enrichment for tyrosine hydroxylase (TH) gene expression in the microdissected sample relative to midbrain sections was approximately 200-fold. For the dopamine transporter (DAT) and the vesicular monoamine transporter type 2 (Vmat2), average enrichments were approximately 100- and 60-fold, respectively. Glutamic acid decarboxylase (Gad65) expression, a marker for GABAergic neurons, was several hundredfold lower than dopamine neuron-specific genes. Glial cell and glutamatergic neuron gene expression were not detected in microdissected samples. Additionally, SN and VTA dopamine neurons had significantly different expression levels of dopamine neuron-specific genes, which likely reflects functional differences between the two cell groups. This study demonstrates that it is possible to laser-microdissect dopamine neurons to a high degree of cell purity. Therefore gene expression profiles can be precisely attributed to the targeted microdissected cells.
Language eng
DOI 10.1155/2013/747938
Field of Research 060603 Animal Physiology - Systems
060805 Animal Neurobiology
110903 Central Nervous System
Socio Economic Objective 920111 Nervous System and Disorders
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2013, Hindawi Publishing Corporation
Persistent URL http://hdl.handle.net/10536/DRO/DU:30062623

Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in TR Web of Science
Scopus Citation Count Cited 7 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 177 Abstract Views, 35 File Downloads  -  Detailed Statistics
Created: Mon, 28 Apr 2014, 10:07:22 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.