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RNAi mediated Tiam1 gene knockdown inhibits invasion of retinoblastoma

Subramanian, Nithya, Navaneethakrishnan, Saranya, Biswas, Jyotirmay, Kanwar, Rupinder K., Kanwar, Jagat R. and Krishnakumar, Subramanian 2013, RNAi mediated Tiam1 gene knockdown inhibits invasion of retinoblastoma, PLoS One, vol. 8, no. 8, pp. e70422.

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Title RNAi mediated Tiam1 gene knockdown inhibits invasion of retinoblastoma
Author(s) Subramanian, Nithya
Navaneethakrishnan, Saranya
Biswas, Jyotirmay
Kanwar, Rupinder K.
Kanwar, Jagat R.
Krishnakumar, Subramanian
Journal name PLoS One
Volume number 8
Issue number 8
Start page e70422
Total pages 14
Publisher PLoS
Place of publication San Francisco, CA
Publication date 2013-08
ISSN 1932-6203
Summary T lymphoma invasion and metastasis protein (Tiam1) is up-regulated in variety of cancers and its expression level is related to metastatic potential of the type of cancer. Earlier, Tiam1 was shown to be overexpressed in retinoblastoma (RB) and we hypothesized that it was involved in invasiveness of RB. This was tested by silencing Tiam1 in RB cell lines (Y79 and Weri-Rb1) using siRNA pool, targeting different regions of Tiam1 mRNA. The cDNA microarray of Tiam1 silenced cells showed gene regulations altered by Tiam1 were predominantly on the actin cytoskeleton interacting proteins, apoptotic initiators and tumorogenic potential targets. The silenced phenotype resulted in decreased growth and increased apoptosis with non-invasive characteristics. Transfection of full length and N-terminal truncated construct (C1199) clearly revealed membrane localization of Tiam1 and not in the case of C580 construct. F-actin staining showed the interaction of Tiam1 with actin in the membrane edges that leads to ruffling, and also imparts varying invasive potential to the cell. The results obtained from our study show for the first time that Tiam1 modulates the cell invasion, mediated by actin cytoskeleton remodeling in RB.
Language eng
Field of Research 111204 Cancer Therapy (excl Chemotherapy and Radiation Therapy)
111207 Molecular Targets
Socio Economic Objective 920102 Cancer and Related Disorders
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2013, PLoS
Persistent URL http://hdl.handle.net/10536/DRO/DU:30062764

Document type: Journal Article
Collections: School of Medicine
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Created: Wed, 30 Apr 2014, 16:44:24 EST by Rupinder Kanwar

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.