Antiarthritic and chondroprotective activity of Lakshadi Guggul in novel alginate-enclosed chitosan calcium phosphate nanocarriers

Samarasinghe, Rasika M., Kanwar, Rupinder K., Kumar, Kuldeep and Kanwar, Jagat R. 2014, Antiarthritic and chondroprotective activity of Lakshadi Guggul in novel alginate-enclosed chitosan calcium phosphate nanocarriers, Nanomedicine, vol. 9, no. 6, pp. 819-837, doi: 10.2217/nnm.13.219.

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Title Antiarthritic and chondroprotective activity of Lakshadi Guggul in novel alginate-enclosed chitosan calcium phosphate nanocarriers
Author(s) Samarasinghe, Rasika M.ORCID iD for Samarasinghe, Rasika M. orcid.org/0000-0002-3669-0985
Kanwar, Rupinder K.
Kumar, Kuldeep
Kanwar, Jagat R.ORCID iD for Kanwar, Jagat R. orcid.org/0000-0003-3728-9568
Journal name Nanomedicine
Volume number 9
Issue number 6
Start page 819
End page 837
Total pages 19
Publisher Future Medicine
Place of publication London, England
Publication date 2014-05
ISSN 1743-5889
Keyword(s) Lakshadi Guggul
antiarthritic and chondroprotective potential
arthritis
rheumatic diseases
Summary Aim
This study aimed to evaluate the antiarthritic and chondroprotective potentials of Lakshadi Guggul (LG) and Cissus quadrangularis encapsulated in novel alginate-enclosed chitosan-calcium phosphate nanocarriers (NCs) both in vitro in primary human chondrocytes and in vivo in mice with collagen-induced arthritis.

Materials & methods:
Chondrocytes exposed to IL-1beta and osteoarthritis chondrocytes grown in an ex vivo inflammation-based coculture were incubated with different concentrations of herbals, and cell modulatory activities were determined. For in vivo studies, herbals and their encapsulated nanoformulations were administered orally to DBA/1 mice with collagen-induce arthritis.

Results:
C. quadrangularis and LG showed enhanced chondroprotective and proliferative activity in IL-1beta-exposed primary chondrocytes, with LG showing the highest therapeutic potency. LG increased viability, proliferative and mitogenic activity, and inhibited cell apoptosis and mitochondrial depolarization. In vivo studies with LG and alginate-enclosed chitosan-calcium phosphate LG NCs revealed cartilage regenerative activity in those administered with the nanoformulation. The NCs were nontoxic to mice, reduced joint swelling and paw volume, and inhibited gene expression of MMPs and cytokines.

Conclusion:
The promising results from this study reveal, for the first time, the novel polymeric NC encapsulating LG as a potential therapeutic for rheumatic diseases. Original submitted 10 October 2013; Revised submitted 13 December 2013.
Language eng
DOI 10.2217/nnm.13.219
Field of Research 110322 Rheumatology and Arthritis
100709 Nanomedicine
110499 Complementary and Alternative Medicine not elsewhere classified
Socio Economic Objective 920116 Skeletal System and Disorders (incl. Arthritis)
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2014, Future Medicine
Persistent URL http://hdl.handle.net/10536/DRO/DU:30062765

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
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Created: Wed, 30 Apr 2014, 17:00:17 EST by Rupinder Kanwar

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