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Rapid development of non-alcoholic steatohepatitis in Psammomys obesus (Israeli Sand Rat)

Spolding, Briana, Connor, Timothy, Wittmer, Carrie, Abreu, Lelia L.F., Kaspi, Antony, Ziemann, Mark, Kaur, Gunveen, Cooper, Adrian, Morrison, Shona, Lee, Scott, Sinclair, Andrew, Gibert, Yann, Trevaskis, James L., Roth, Jonathon D., El-Osta, Assam, Standish, Richard and Walder, Ken 2014, Rapid development of non-alcoholic steatohepatitis in Psammomys obesus (Israeli Sand Rat), PLoS one, vol. 9, no. 3, pp. 1-7.

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Title Rapid development of non-alcoholic steatohepatitis in Psammomys obesus (Israeli Sand Rat)
Author(s) Spolding, Briana
Connor, Timothy
Wittmer, Carrie
Abreu, Lelia L.F.
Kaspi, Antony
Ziemann, Mark
Kaur, Gunveen
Cooper, Adrian
Morrison, Shona
Lee, Scott
Sinclair, Andrew
Gibert, Yann
Trevaskis, James L.
Roth, Jonathon D.
El-Osta, Assam
Standish, Richard
Walder, Ken
Journal name PLoS one
Volume number 9
Issue number 3
Start page 1
End page 7
Total pages 7
Publisher Public Library of Science
Place of publication San Francisco, Calif.
Publication date 2014
ISSN 1932-6203
Summary Background and AimsA major impediment to establishing new treatments for non-alcoholic steatohepatitis is the lack of suitable animal models that accurately mimic the biochemical and metabolic characteristics of the disease. The aim of this study was to explore a unique polygenic animal model of metabolic disease as a model of non-alcoholic steatohepatitis by determining the effects of 2% dietary cholesterol supplementation on metabolic and liver endpoints in Psammomys obesus (Israeli sand rat).MethodsP. obesus were provided ad libitum access to either a standard rodent diet (20% kcal/fat) or a standard rodent diet supplemented with 2% cholesterol (w/w) for 4 weeks. Histological sections of liver from animals on both diets were examined for key features of non-alcoholic steatohepatitis. The expression levels of key genes involved in hepatic lipid metabolism were measured by real-time PCR.ResultsP. obesus fed a cholesterol-supplemented diet exhibited profound hepatomegaly and steatosis, and higher plasma transaminase levels. Histological analysis identified extensive steatosis, inflammation, hepatocyte injury and fibrosis. Hepatic gene expression profiling revealed decreased expression of genes involved in delivery and uptake of lipids, and fatty acid and triglyceride synthesis, and increased expression of genes involved in very low density lipoprotein cholesterol synthesis, triglyceride and cholesterol export.ConclusionsP. obesus rapidly develop non-alcoholic steatohepatitis when fed a cholesterol-supplemented diet that appears to be histologically and mechanistically similar to patients.
Language eng
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2014, Public Library of Science
Persistent URL http://hdl.handle.net/10536/DRO/DU:30063544

Document type: Journal Article
Collections: School of Medicine
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