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Expression of muscarinic receptor subtypes in tree shrew ocular tissues and their regulation during the development of myopia

McBrien, N.A., Jobling, A.I., Truong, H.T., Cottriall, C.L. and Gentle, A. 2009, Expression of muscarinic receptor subtypes in tree shrew ocular tissues and their regulation during the development of myopia, Molecular vision: Biology and genetics in vision research, vol. 15, pp. 464-475.

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Title Expression of muscarinic receptor subtypes in tree shrew ocular tissues and their regulation during the development of myopia
Author(s) McBrien, N.A.
Jobling, A.I.
Truong, H.T.
Cottriall, C.L.
Gentle, A.ORCID iD for Gentle, A. orcid.org/0000-0003-0661-4196
Journal name Molecular vision: Biology and genetics in vision research
Volume number 15
Start page 464
End page 475
Total pages 12
Publisher PubMed Central
Place of publication Atlanta, Ga.
Publication date 2009
ISSN 1090-0535
Summary Muscarinic receptors are known to regulate several important physiologic processes in the eye. Antagonists to these receptors such as atropine and pirenzepine are effective at stopping the excessive ocular growth that results in myopia. However, their site of action is unknown. This study details ocular muscarinic subtype expression within a well documented model of eye growth and investigates their expression during early stages of myopia induction. Total RNA was isolated from tree shrew corneal, iris/ciliary body, retinal, choroidal, and scleral tissue samples and was reverse transcribed. Using tree shrew-specific primers to the five muscarinic acetylcholine receptor subtypes (CHRM1-CHRM5), products were amplified using polymerase chain reaction (PCR) and their identity confirmed using automated sequencing. The expression of the receptor proteins (M1-M5) were also explored in the retina, choroid, and sclera using immunohistochemistry. Myopia was induced in the tree shrew for one or five days using monocular deprivation of pattern vision, and the expression of the receptor subtypes was assessed in the retina, choroid, and sclera using real-time PCR. All five muscarinic receptor subtypes were expressed in the iris/ciliary body, retina, choroid, and sclera while gene products corresponding to CHRM1, CHRM3, CHRM4, and CHRM5 were present in the corneal samples. The gene expression data were confirmed by immunohistochemistry with the M1-M5 proteins detected in the retina, choroid, and sclera. After one or five days of myopia development, muscarinic receptor gene expression remained unaltered in the retinal, choroidal, and scleral tissue samples. This study provides a comprehensive profile of muscarinic receptor gene and protein expression in tree shrew ocular tissues with all receptor subtypes found in tissues implicated in the control of eye growth. Despite the efficacy of muscarinic antagonists at inhibiting myopia development, the genes of the muscarinic receptor subtypes are neither regulated early in myopia (before measurable axial elongation) nor after significant structural change.
Language eng
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2009, PubMed Central
Persistent URL http://hdl.handle.net/10536/DRO/DU:30064341

Document type: Journal Article
Collections: School of Medicine
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.