The A622 gene in Nicotiana glauca (tree tobacco): evidence for a functional role in pyridine alkaloid synthesis DeBoer, Kathleen D., Lye, Jessica C., Aitkin, Campbell D., Su, Angela K.K. and Hamill, John D. 2009, The A622 gene in Nicotiana glauca (tree tobacco): evidence for a functional role in pyridine alkaloid synthesis, Plant molecular biology, vol. 69, no. 3, pp. 299-312, doi: 10.1007/s11103-008-9425-2. Attached Files Name Description MIMEType Size Downloads Title The A622 gene in Nicotiana glauca (tree tobacco): evidence for a functional role in pyridine alkaloid synthesis Author(s) DeBoer, Kathleen D. Lye, Jessica C. Aitkin, Campbell D. Su, Angela K.K. Hamill, John D. Journal name Plant molecular biology Volume number 69 Issue number 3 Start page 299 End page 312 Total pages 14 Publisher Springer-Verlag Place of publication Berlin, Germany Publication date 2009-02 ISSN 1573-5028 Keyword(s) anabasine anatabine hairy roots nicotine RNAi transgenic Summary Nicotiana glauca (Argentinean tree tobacco) is atypical within the genus Nicotiana, accumulating predominantly anabasine rather than nicotine and/or nornicotine as the main component of its leaf pyridine alkaloid fraction. The current study examines the role of the A622 gene from N. glauca (NgA622) in alkaloid production and utilises an RNAi approach to down-regulate gene expression and diminish levels of A622 protein in transgenic tissues. Results indicate that RNAi-mediated reduction in A622 transcript levels markedly reduces the capacity of N. glauca to produce anabasine resulting in plants with scarcely any pyridine alkaloids in leaf tissues, even after damage to apical tissues. In addition, analysis of hairy roots containing the NgA622-RNAi construct shows a substantial reduction in both anabasine and nicotine levels within these tissues, even if stimulated with methyl jasmonate, indicating a role for the A622 enzyme in the synthesis of both alkaloids in roots of N. glauca. Feeding of Nicotinic Acid (NA) to hairy roots of N. glauca containing the NgA622-RNAi construct did not restore capacity for synthesis of anabasine or nicotine. Moreover, treatment of these hairy root lines with NA did not lead to an increase in anatabine levels, unlike controls. Together, these results strongly suggest that A622 is an integral component of the final enzyme complex responsible for biosynthesis of all three pyridine alkaloids in Nicotiana. Language eng DOI 10.1007/s11103-008-9425-2 Field of Research 059999 Environmental Sciences not elsewhere classified Socio Economic Objective 970105 Expanding Knowledge in the Environmental Sciences HERDC Research category C1.1 Refereed article in a scholarly journal Persistent URL http://hdl.handle.net/10536/DRO/DU:30064358 Document type: Journal Article Collection: Office of the Deputy Vice-Chancellor Research Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Fri, 13 Jun 2014, 12:05:00 EST Mon, 16 Jun 2014, 13:40:43 EST Fri, 10 Oct 2014, 09:21:07 EST Thu, 11 Dec 2014, 20:36:03 EST Filtered Full Citation counts:   Cited 43 times in TR Web of Science Cited 50 times in Scopus Search Google Scholar Access Statistics: 140 Abstract Views, 1 File Downloads  -  Detailed Statistics Created: Fri, 13 Jun 2014, 12:05:00 EST

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