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Increased biomass, seed yield and stress tolerance is conferred in Arabidopsis by a novel enzyme from the resurrection grass Sporobolus stapfianus that glycosylates the strigolactone analogue GR24

Islam, Sharmin, Griffiths, Cara A., Blomstedt, Cecilia K., Le, Tuan-Ngoc, Gaff, Donald F., Hamill, John D. and Neale, Alan D. 2013, Increased biomass, seed yield and stress tolerance is conferred in Arabidopsis by a novel enzyme from the resurrection grass Sporobolus stapfianus that glycosylates the strigolactone analogue GR24, PLoS one, vol. 8, no. 11, pp. 1-12, doi: 10.1371/journal.pone.0080035.

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Title Increased biomass, seed yield and stress tolerance is conferred in Arabidopsis by a novel enzyme from the resurrection grass Sporobolus stapfianus that glycosylates the strigolactone analogue GR24
Formatted title Increased biomass, seed yield and stress tolerance is conferred in Arabidopsis by a novel enzyme from the resurrection grass Sporobolus stapfianus that glycosylates the strigolactone analogue GR24
Author(s) Islam, Sharmin
Griffiths, Cara A.
Blomstedt, Cecilia K.
Le, Tuan-Ngoc
Gaff, Donald F.
Hamill, John D.
Neale, Alan D.
Journal name PLoS one
Volume number 8
Issue number 11
Article ID e80035
Start page 1
End page 12
Total pages 12
Publisher Public Library of Science
Place of publication San Francisco, Calif.
Publication date 2013
ISSN 1932-6203
Summary Isolation of gene transcripts from desiccated leaf tissues of the resurrection grass, Sporobolus stapfianus, resulted in the identification of a gene, SDG8i, encoding a Group 1 glycosyltransferase (UGT). Here, we examine the effects of introducing this gene, under control of the CaMV35S promoter, into the model plant Arabidopsis thaliana. Results show that Arabidopsis plants constitutively over-expressing SDG8i exhibit enhanced growth, reduced senescence, cold tolerance and a substantial improvement in protoplasmic drought tolerance. We hypothesise that expression of SDG8i in Arabidopsis negatively affects the bioactivity of metabolite/s that mediate/s environmentally-induced repression of cell division and expansion, both during normal development and in response to stress. The phenotype of transgenic plants over-expressing SDG8i suggests modulation in activities of both growth- and stress-related hormones. Plants overexpressing the UGT show evidence of elevated auxin levels, with the enzyme acting downstream of ABA to reduce drought-induced senescence. Analysis of the in vitro activity of the UGT recombinant protein product demonstrates that SDG8i can glycosylate the synthetic strigolactone analogue GR24, evoking a link with strigolactone-related processes in vivo. The large improvements observed in survival of transgenic Arabidopsis plants under cold-, salt- and drought-stress, as well as the substantial increases in growth rate and seed yield under non-stress conditions, indicates that overexpression of SDG8i in crop plants may provide a novel means of increasing plant productivity.
Language eng
DOI 10.1371/journal.pone.0080035
Field of Research 049999 Earth Sciences not elsewhere classified
Socio Economic Objective 970104 Expanding Knowledge in the Earth Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2013, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30064362

Document type: Journal Article
Collections: Office of the Deputy Vice-Chancellor Research
Open Access Collection
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.