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An interspecific Nicotiana hybrid as a useful and cost-effective platform for production of animal vaccines

Ling, Huai-Yian, Edwards, Aaron M., Gantier, Michael P., DeBoer, Kathleen D., Neale, Alan D., Hamill, John D. and Walmsley, Amanda M. 2012, An interspecific Nicotiana hybrid as a useful and cost-effective platform for production of animal vaccines, PLoS one, vol. 7, no. 4, pp. 1-14, doi: 10.1371/journal.pone.0035688.

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Title An interspecific Nicotiana hybrid as a useful and cost-effective platform for production of animal vaccines
Formatted title An interspecific Nicotiana hybrid as a useful and cost-effective platform for production of animal vaccines
Author(s) Ling, Huai-Yian
Edwards, Aaron M.
Gantier, Michael P.
DeBoer, Kathleen D.
Neale, Alan D.
Hamill, John D.
Walmsley, Amanda M.
Journal name PLoS one
Volume number 7
Issue number 4
Article ID e35688
Start page 1
End page 14
Total pages 14
Publisher Public Library of Science
Place of publication San Francisco, Calif.
Publication date 2012
ISSN 1932-6203
Summary The use of transgenic plants to produce novel products has great biotechnological potential as the relatively inexpensive inputs of light, water, and nutrients are utilised in return for potentially valuable bioactive metabolites, diagnostic proteins and vaccines. Extensive research is ongoing in this area internationally with the aim of producing plant-made vaccines of importance for both animals and humans. Vaccine purification is generally regarded as being integral to the preparation of safe and effective vaccines for use in humans. However, the use of crude plant extracts for animal immunisation may enable plant-made vaccines to become a cost-effective and efficacious approach to safely immunise large numbers of farm animals against diseases such as avian influenza. Since the technology associated with genetic transformation and large-scale propagation is very well established in Nicotiana, the genus has attributes well-suited for the production of plant-made vaccines. However the presence of potentially toxic alkaloids in Nicotiana extracts impedes their use as crude vaccine preparations. In the current study we describe a Nicotiana tabacum and N. glauca hybrid that expresses the HA glycoprotein of influenza A in its leaves but does not synthesize alkaloids. We demonstrate that injection with crude leaf extracts from these interspecific hybrid plants is a safe and effective approach for immunising mice. Moreover, this antigen-producing alkaloid-free, transgenic interspecific hybrid is vigorous, with a high capacity for vegetative shoot regeneration after harvesting. These plants are easily propagated by vegetative cuttings and have the added benefit of not producing viable pollen, thus reducing potential problems associated with bio-containment. Hence, these Nicotiana hybrids provide an advantageous production platform for partially purified, plant-made vaccines which may be particularly well suited for use in veterinary immunization programs.
Language eng
DOI 10.1371/journal.pone.0035688
Field of Research 079999 Agricultural and Veterinary Sciences not elsewhere classified
Socio Economic Objective 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2012, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30064364

Document type: Journal Article
Collections: Office of the Deputy Vice-Chancellor Research
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.