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APETALA2/ETHYLENE respnse factor and basic helix-loop-helix tobacco transcription factors cooperatively mediate jasmonate-elicited nicotine biosynthesis.

De, Boer Kathleen, Tilleman, Sofie, Pauwels, Laurens, Vanden, Bossche Robin, De, Sutter Valerie, Vanderhaeghen, Rudy. Hilson Pierre, Hamill, John D. and Goossens, Alain 2011, APETALA2/ETHYLENE respnse factor and basic helix-loop-helix tobacco transcription factors cooperatively mediate jasmonate-elicited nicotine biosynthesis., Plant journal: for cell and molecular biology, vol. 66, no. 6, pp. 1053-1065, doi: 10.1111/j.1365-313X.2011.04566.x.

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Title APETALA2/ETHYLENE respnse factor and basic helix-loop-helix tobacco transcription factors cooperatively mediate jasmonate-elicited nicotine biosynthesis.
Author(s) De, Boer Kathleen
Tilleman, Sofie
Pauwels, Laurens
Vanden, Bossche Robin
De, Sutter Valerie
Vanderhaeghen, Rudy. Hilson Pierre
Hamill, John D.
Goossens, Alain
Journal name Plant journal: for cell and molecular biology
Volume number 66
Issue number 6
Start page 1053
End page 1065
Total pages 13
Publisher Blackwell
Place of publication Oxford, Eng.
Publication date 2011-06
ISSN 1365-313X
Keyword(s) Nicotiana
basic helix–loop–helix
ethylene response factor
nicotine
jasmonate
mitogen-activated protein kinase
Summary Transcription factors of the plant-specific apetala2/ethylene response factor (AP2/ERF) family control plant secondary metabolism, often as part of signalling cascades induced by jasmonate (JA) or other elicitors. Here, we functionally characterized the JA-inducible tobacco (Nicotiana tabacum) AP2/ERF factor ORC1, one of the members of the NIC2-locus ERFs that control nicotine biosynthesis and a close homologue of ORCA3, a transcriptional activator of alkaloid biosynthesis in Catharanthus roseus. ORC1 positively regulated the transcription of several structural genes coding for the enzymes involved in nicotine biosynthesis. Accordingly, overexpression of ORC1 was sufficient to stimulate alkaloid biosynthesis in tobacco plants and tree tobacco (Nicotiana glauca) root cultures. In contrast to ORCA3 in C. roseus, which needs only the GCC motif in the promoters of the alkaloid synthesis genes to induce their expression, ORC1 required the presence of both GCC-motif and G-box elements in the promoters of the tobacco nicotine biosynthesis genes for maximum transactivation. Correspondingly, combined application with the JA-inducible Nicotiana basic helix–loop–helix (bHLH) factors that bind the G-box element in these promoters enhanced ORC1 action. Conversely, overaccumulation of JAZ repressor proteins that block bHLH activity reduced ORC1 functionality. Finally, the activity of both ORC1 and bHLH proteins was post-translationally upregulated by a JA-modulated phosphorylation cascade, in which a specific mitogen-activated protein kinase kinase, JA-factor stimulating MAPKK1 (JAM1), was identified. This study highlights the complexity of the molecular machinery involved in the regulation of tobacco alkaloid biosynthesis and provides mechanistic insights about its transcriptional regulators.
Language eng
DOI 10.1111/j.1365-313X.2011.04566.x
Field of Research 039999 Chemical Sciences not elsewhere classified
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30064384

Document type: Journal Article
Collections: Office of the Deputy Vice-Chancellor (Research)
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.