You are not logged in.

Use of the wound-inducible NtQPT2 promoter from Nicotiana tabacum for production of a plant-made vaccine

De, Guzman Giorgio, Walmsley, Amanda M., Webster, Diane E. and Hamill, John D. 2012, Use of the wound-inducible NtQPT2 promoter from Nicotiana tabacum for production of a plant-made vaccine, Biotechnology letters, vol. 34, no. 6, pp. 1143-1150, doi: 10.1007/s10529-012-0879-6.

Attached Files
Name Description MIMEType Size Downloads

Title Use of the wound-inducible NtQPT2 promoter from Nicotiana tabacum for production of a plant-made vaccine
Author(s) De, Guzman Giorgio
Walmsley, Amanda M.
Webster, Diane E.
Hamill, John D.
Journal name Biotechnology letters
Volume number 34
Issue number 6
Start page 1143
End page 1150
Total pages 8
Publisher Kluwer Academic Publishers
Place of publication Amsterdam, The Netherlands
Publication date 2012-06
ISSN 1573-6776
Keyword(s) plants
genetically modified
promoter regions
genetic
tobacco
vaccines
bacterial toxins
Summary The wound-inducible quinolinate phosphoribosyl transferase promoter from Nicotiana tabacum (NtQPT2) was assessed for its capacity to produce B-subunit of the heat-labile toxin (LTB) from enterotoxigenic Escherichia coli in transgenic plant tissues. Comparisons were made with the widely used and constitutive Cauliflower Mosaic Virus 35S (CaMV35S) promoter. The NtQPT2 promoter produced somewhat lower average concentrations of LTB protein per unit weight of hairy root tissue but allowed better growth thereby producing similar or higher overall average yields of LTB per culture batch. Transgenic tobacco plants containing the NtQPT2-LTB construct contained LTB protein in roots but not leaves. Moreover, wounding NtQPT2-LTB transgenic plants, by removal of apices, resulted in an approximate 500% increase in LTB levels in roots when analysed several days later. CaMV35S-LTB transgenic plants contained LTB protein in leaves and roots but wounding made no difference to their LTB content.
Language eng
DOI 10.1007/s10529-012-0879-6
Field of Research 039999 Chemical Sciences not elsewhere classified
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2012, Kluwer Academic Publishers
Persistent URL http://hdl.handle.net/10536/DRO/DU:30064403

Document type: Journal Article
Collection: Office of the Deputy Vice-Chancellor Research
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 1 times in TR Web of Science
Scopus Citation Count Cited 3 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 100 Abstract Views, 1 File Downloads  -  Detailed Statistics
Created: Fri, 13 Jun 2014, 12:11:31 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.