Use of the wound-inducible NtQPT2 promoter from Nicotiana tabacum for production of a plant-made vaccine De, Guzman Giorgio, Walmsley, Amanda M., Webster, Diane E. and Hamill, John D. 2012, Use of the wound-inducible NtQPT2 promoter from Nicotiana tabacum for production of a plant-made vaccine, Biotechnology letters, vol. 34, no. 6, pp. 1143-1150, doi: 10.1007/s10529-012-0879-6. Attached Files Name Description MIMEType Size Downloads Title Use of the wound-inducible NtQPT2 promoter from Nicotiana tabacum for production of a plant-made vaccine Author(s) De, Guzman Giorgio Walmsley, Amanda M. Webster, Diane E. Hamill, John D. Journal name Biotechnology letters Volume number 34 Issue number 6 Start page 1143 End page 1150 Total pages 8 Publisher Kluwer Academic Publishers Place of publication Amsterdam, The Netherlands Publication date 2012-06 ISSN 1573-6776 Keyword(s) plants genetically modified promoter regions genetic tobacco vaccines bacterial toxins Summary The wound-inducible quinolinate phosphoribosyl transferase promoter from Nicotiana tabacum (NtQPT2) was assessed for its capacity to produce B-subunit of the heat-labile toxin (LTB) from enterotoxigenic Escherichia coli in transgenic plant tissues. Comparisons were made with the widely used and constitutive Cauliflower Mosaic Virus 35S (CaMV35S) promoter. The NtQPT2 promoter produced somewhat lower average concentrations of LTB protein per unit weight of hairy root tissue but allowed better growth thereby producing similar or higher overall average yields of LTB per culture batch. Transgenic tobacco plants containing the NtQPT2-LTB construct contained LTB protein in roots but not leaves. Moreover, wounding NtQPT2-LTB transgenic plants, by removal of apices, resulted in an approximate 500% increase in LTB levels in roots when analysed several days later. CaMV35S-LTB transgenic plants contained LTB protein in leaves and roots but wounding made no difference to their LTB content. Language eng DOI 10.1007/s10529-012-0879-6 Field of Research 039999 Chemical Sciences not elsewhere classified Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences HERDC Research category C1.1 Refereed article in a scholarly journal Copyright notice ©2012, Kluwer Academic Publishers Persistent URL http://hdl.handle.net/10536/DRO/DU:30064403 Document type: Journal Article Collection: Office of the Deputy Vice-Chancellor Research Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Fri, 13 Jun 2014, 12:11:31 EST Mon, 16 Jun 2014, 08:30:40 EST Fri, 20 Jun 2014, 14:46:21 EST Fri, 10 Oct 2014, 09:21:44 EST Thu, 11 Dec 2014, 20:36:56 EST Filtered Full Citation counts:   Cited 1 times in TR Web of Science Cited 3 times in Scopus Search Google Scholar Access Statistics: 89 Abstract Views, 1 File Downloads  -  Detailed Statistics Created: Fri, 13 Jun 2014, 12:11:31 EST

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