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Inhibition of matrix metalloproteinase activity in the chick sclera and its effect on myopia development

Liu, Hsin-Hua, Gentle, Alex, Jobling, Andrew I. and McBrien, Neville A. 2010, Inhibition of matrix metalloproteinase activity in the chick sclera and its effect on myopia development, Investigative ophthalmology & visual science, vol. 51, no. 6, pp. 2865-2871, doi: 10.1167/iovs.09-4322.

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Title Inhibition of matrix metalloproteinase activity in the chick sclera and its effect on myopia development
Author(s) Liu, Hsin-Hua
Gentle, Alex
Jobling, Andrew I.
McBrien, Neville A.
Journal name Investigative ophthalmology & visual science
Volume number 51
Issue number 6
Start page 2865
End page 2871
Total pages 7
Publisher Association for Research in Vision and Ophtalmology
Place of publication Rockville, Md.
Publication date 2010-06
ISSN 1552-5783
Keyword(s) metalloprotein
matrix metalloprotein
myopia development
Summary To investigate the contribution of matrix degradation in the two-layer avian sclera to the development of myopia. Tissue inhibitor of metalloproteinase-2 (TIMP-2) was used to inhibit chick scleral collagen degradation with (3)H-proline, a marker for this effect. Ex vivo scleral culture experiments confirmed TIMP-2 doses for in vivo experimentation. Ocular growth and refractive response to exogenous TIMP-2 (11.25, 2.25, and 0.45 picomoles, plus vehicle only) were monitored in 7-day-old chicks during the induction of myopia over 4 days with a translucent occluder. Collagen degradation was assessed, in whole sclera and in separated scleral layers by using the same paradigm (11.25 picomoles TIMP-2; vehicle only).Approximately 60% of collagen degradation was inhibited with low (2 nM) doses of TIMP-2 in the ex vivo sclera. Degradative activity in the in vivo chick sclera increased significantly (46%) during myopia development, with all the altered activity confined to the fibrous layer. Addition of TIMP-2 significantly reduced (by 46%) this accelerated scleral collagen degradation, also by acting in the fibrous layer. TIMP-2 had no significant effect on (3)H-proline incorporated in the cartilaginous scleral layer and cornea. Despite inhibiting collagen degradation TIMP-2 had no significant effect on myopia development. Increased collagen degradation is a feature of scleral remodeling in chick myopia development, but is confined to the fibrous scleral layer. Significant inhibition of this collagenolytic activity with TIMP-2 has little effect on refractive error development, suggesting that collagen degradation in the sclera contributes little to the development of myopia in the chick.
Language eng
DOI 10.1167/iovs.09-4322
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2011, Association for Research in Vision and Ophtalmology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30064469

Document type: Journal Article
Collection: School of Medicine
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