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Maternal dietary supplementation with saturated, but not monounsaturated or polyunsaturated fatty acids, leads to tissue-specific inhibition of offspring Na+,K+-ATPase

Armitage, James A., Gupta, Sanjana, Woods, Caroline, Jensen, Runa I., Samuelsson, Anne-Maj, Fuller, William, Shattock, Michael J., Poston, Lucilla and Taylor, Paul D. 2008, Maternal dietary supplementation with saturated, but not monounsaturated or polyunsaturated fatty acids, leads to tissue-specific inhibition of offspring Na+,K+-ATPase, Journal of physiology, vol. 586, no. 20, pp. 5013-5022, doi: 10.1113/jphysiol.2008.157818.

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Title Maternal dietary supplementation with saturated, but not monounsaturated or polyunsaturated fatty acids, leads to tissue-specific inhibition of offspring Na+,K+-ATPase
Author(s) Armitage, James A.
Gupta, Sanjana
Woods, Caroline
Jensen, Runa I.
Samuelsson, Anne-Maj
Fuller, William
Shattock, Michael J.
Poston, Lucilla
Taylor, Paul D.
Journal name Journal of physiology
Volume number 586
Issue number 20
Start page 5013
End page 5022
Total pages 10
Publisher Wiley
Place of publication London, Eng.
Publication date 2008-10
ISSN 0022-3751
1469-7793
Summary In rats, a maternal diet rich in lard is associated with reduced Na+,K+-ATPase activity in adult offspring kidney. We have addressed the role of different fatty acids by evaluating Na+,K+-ATPase activity in offspring of dams fed diets rich in saturated (SFA), monounsaturated (MUFA) or polyunsaturated (PUFA) fatty acids. Female Sprague–Dawley rats were fed, during pregnancy and suckling, a control diet (4% w/w corn oil) or a fatty acid supplemented diet (24% w/w). Offspring were reared on chow (4% PUFA) and studied at 6 months. mRNA expression (real-time PCR) of Na+,K+-ATPase α subunit and protein expression of Na+,K+-ATPase subunits (Western blot) were assessed in kidney and brain. Na+,K+-ATPase activity was reduced in kidney (P < 0.05 versus all groups) and brain (P < 0.05 versus control and MUFA offspring) of the SFA group. Neither Na+,K+-ATPase α1 subunit mRNA expression, nor protein expression of total α, α1, α2, α3 or β1 subunits were significantly altered in kidney in any dietary group. In brains of SFA offspring α1 mRNA expression (P < 0.05) was reduced compared with MUFA and PUFA offspring, but not controls. Also in brain, SFA offspring demonstrated reduced (P < 0.05) α1 subunit protein and increased phosphorylation (P < 0.05) of the Na+,K+-ATPase modulating protein phospholemman at serine residue 63 (S63 PLM). Na+,K+-ATPase activity was similar to controls in heart and liver. In utero and neonatal exposure to a maternal diet rich in saturated fatty acids is associated with altered activity and expression of Na+,K+-ATPase in adulthood, but mechanisms appear tissue specific.
Language eng
DOI 10.1113/jphysiol.2008.157818
Field of Research 179999 Psychology and Cognitive Sciences not elsewhere classified
Socio Economic Objective 970117 Expanding Knowledge in Psychology and Cognitive Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2008, Wiley
Persistent URL http://hdl.handle.net/10536/DRO/DU:30064854

Document type: Journal Article
Collection: School of Medicine
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