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Maternal high-fat diet alters expression of pathways of growth, blood supply and arachidonic acid in rat placenta

Dekker, Nitert Marloes, Vaswani, Kanchan, Hum, Melissa, Chan, Hsiu-Wen, Wood-Bradley, Ryan, Henry, Sarah, Armitage, James A., Mitchell, Murray D. and Rice, Gregory E. 2013, Maternal high-fat diet alters expression of pathways of growth, blood supply and arachidonic acid in rat placenta, Journal of nutrition science, vol. 2, pp. 1-7, doi: 10.1017/jns.2013.36.

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Title Maternal high-fat diet alters expression of pathways of growth, blood supply and arachidonic acid in rat placenta
Author(s) Dekker, Nitert Marloes
Vaswani, Kanchan
Hum, Melissa
Chan, Hsiu-Wen
Wood-Bradley, Ryan
Henry, Sarah
Armitage, James A.
Mitchell, Murray D.
Rice, Gregory E.
Journal name Journal of nutrition science
Volume number 2
Start page 1
End page 7
Total pages 7
Publisher Cambridge University Press
Place of publication Cambridge, Eng.
Publication date 2013
ISSN 2048-6790
Keyword(s) Placenta
High-fat diet
Arachidonic acid
LIM domain kinase 1
Summary The high fat content in Western diets probably affects placental function during pregnancy with potential consequences for the offspring in the short and long term. The aim of the present study was to compare genome-wide placental gene expression between rat dams fed a high-fat diet (HFD) and those fed a control diet for 3 weeks before conception and during gestation. Gene expression was measured by microarray and pathway analysis was performed. Gene expression differences were replicated by real-time PCR and protein expression was assessed by Western blot analysis. Placental and fetal weights at E17.25 were not altered by exposure to the maternal HFD. Gene pathways targeting placental growth, blood supply and chemokine signalling were up-regulated in the placentae of dams fed the HFD. The up-regulation in messenger RNA expression for five genes Ptgs2 (fatty acid cyclo-oxidase 2; COX2), Limk1 (LIM domain kinase 1), Pla2g2a (phospholipase A2), Itga1 (integrin α-1) and Serpine1 was confirmed by real-time PCR. Placental protein expression for COX2 and LIMK was also increased in HFD-fed dams. In conclusion, maternal HFD feeding alters placental gene expression patterns of placental growth and blood supply and specifically increases the expression of genes involved in arachidonic acid and PG metabolism. These changes indicate a placental response to the altered maternal metabolic environment.
Language eng
DOI 10.1017/jns.2013.36
Field of Research 179999 Psychology and Cognitive Sciences not elsewhere classified
Socio Economic Objective 970117 Expanding Knowledge in Psychology and Cognitive Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2013, Cambridge University Press
Persistent URL http://hdl.handle.net/10536/DRO/DU:30064859

Document type: Journal Article
Collections: School of Medicine
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.