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A design-based method for estimating glomerular number in the developing kidney

Cullen-McEwen, Luise A., Armitage, James A., Nyengaard, Jens R., Moritz, Karen M. and Bertram, John F. 2011, A design-based method for estimating glomerular number in the developing kidney, Renal physiology, vol. 300, pp. 1448-1453, doi: 10.1152/ajprenal.00055.2011.

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Title A design-based method for estimating glomerular number in the developing kidney
Author(s) Cullen-McEwen, Luise A.
Armitage, James A.
Nyengaard, Jens R.
Moritz, Karen M.
Bertram, John F.
Journal name Renal physiology
Volume number 300
Start page 1448
End page 1453
Total pages 6
Publisher Biomedical Information Project, University of Sheffield
Place of publication Sheffield, Eng.
Publication date 2011
ISSN 0300-3434
Summary Low glomerular (nephron) endowment has been associated with an increased risk of cardiovascular and renal disease in adulthood. Nephron endowment in humans is determined by 36 wk of gestation, while in rats and mice nephrogenesis ends several days after birth. Specific genes and environmental perturbations have been shown to regulate nephron endowment. Until now, design-based method for estimating nephron number in developing kidneys was unavailable. This was due in part to the difficulty associated with unambiguously identifying developing glomeruli in histological sections. Here, we describe a method that uses lectin histochemistry to identify developing glomeruli and the physical disector/fractionator principle to provide unbiased estimates of total glomerular number (Nglom). We have characterized Nglom throughout development in kidneys from 76 rats and model this development with a 5-parameter logistic equation to predict Nglom from embryonic day 17.25 to adulthood (r2 = 0.98). This approach represents the first design-based method with which to estimate Nglom in the developing kidney.
Language eng
DOI 10.1152/ajprenal.00055.2011
Field of Research 179999 Psychology and Cognitive Sciences not elsewhere classified
Socio Economic Objective 970117 Expanding Knowledge in Psychology and Cognitive Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2011, Biomedical Information Project, University of Sheffield
Persistent URL http://hdl.handle.net/10536/DRO/DU:30064861

Document type: Journal Article
Collection: School of Medicine
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