Improved efficacy and reduced toxicity of doxorubicin encapsulated in sulfatide-containing nanoliposome in a glioma model Lin, Jia, Shigdar, Sarah, Fang, Ding Zhi, Xiang, Dognxi, Wei, Ming Q., Danks, Andrew, Kong, Lingxue, Li, Lianghong, Qiao, Liang and Duan, Wei 2014, Improved efficacy and reduced toxicity of doxorubicin encapsulated in sulfatide-containing nanoliposome in a glioma model, Plos One, vol. 9, no. 7, e103736, pp. 1-13, doi: 10.1371/journal.pone.0103736. Attached Files Name Description MIMEType Size Downloads lin-improvedefficacy-2014.pdf Published version application/pdf 721.05KB 59 Title Improved efficacy and reduced toxicity of doxorubicin encapsulated in sulfatide-containing nanoliposome in a glioma model Author(s) Lin, Jia Shigdar, Sarah Fang, Ding Zhi Xiang, Dognxi Wei, Ming Q. Danks, Andrew Kong, Lingxue orcid.org/0000-0001-6219-3897 Li, Lianghong Qiao, Liang Duan, Wei Journal name Plos One Volume number 9 Issue number 7 Season e103736 Start page 1 End page 13 Total pages 13 Publisher Public Library of Science Place of publication San Francisco, CA Publication date 2014-07 ISSN 1932-6203 Summary As a glycosphingolipid that can bind to several extracellular matrix proteins, sulfatide has the potential to become an effective targeting agent for tumors overexpressing tenasin-C in their microenvironment. To overcome the dose-limiting toxicity of doxorubicin (DOX), a sulfatide-containing nanoliposome (SCN) encapsulation approach was employed to improve treatment efficacy and reduce side effects of free DOX. This study analysed in vitro characteristics of sulfatidecontaining nanoliposomal DOX (SCN-DOX) and assessed its cytotoxicity in vitro, as well as biodistribution, therapeutic efficacy, and systemic toxicity in a human glioblastoma U-118MG xenograft model. SCN-DOX was shown to achieve highest drug to lipid ratio (0.5:1) and a remarkable in vitro stability. Moreover, DOX encapsulated in SCN was shown to be delivered into the nuclei and displayed prolonged retention over free DOX in U-118MG cells. This simple two-lipid SCN- DOX nanodrug has favourable pharmacokinetic attributes in terms of prolonged circulation time, reduced volume of distribution and enhanced bioavailability in healthy rats. As a result of the improved biodistribution, an enhanced treatment efficacy of SCNDOX was found in glioma-bearing mice compared to the free drug. Finally, a reduction in the accumulation of DOX in the drug’s principal toxicity organs achieved by SCN-DOX led to the diminished systemic toxicity as evident from the plasma biochemical analyses. Thus, SCN has the potential to be an effective and safer nano-carrier for targeted delivery of therapeutic agents to tumors with elevated expression of tenascin-C in their microenvironment. Language eng DOI 10.1371/journal.pone.0103736 Field of Research 111504 Pharmaceutical Sciences Socio Economic Objective 920102 Cancer and Related Disorders HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2014, Public Library of Science Free to Read? Yes Use Rights Persistent URL http://hdl.handle.net/10536/DRO/DU:30065417 Document type: Journal Article Collections: School of Medicine Open Access Collection Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Thu, 14 Aug 2014, 15:35:40 EST Fri, 15 Aug 2014, 09:16:11 EST Fri, 22 Aug 2014, 08:34:23 EST Fri, 22 Aug 2014, 08:37:01 EST Wed, 05 Nov 2014, 11:17:05 EST Thu, 06 Nov 2014, 09:18:05 EST Fri, 12 Dec 2014, 11:02:06 EST Mon, 19 Jan 2015, 12:32:17 EST Fri, 26 Aug 2016, 15:15:53 EST Mon, 05 Sep 2016, 16:03:32 EST Wed, 07 Sep 2016, 07:34:44 EST Filtered Full Citation counts:   Cited 4 times in TR Web of Science Cited 5 times in Scopus Search Google Scholar Access Statistics: 214 Abstract Views, 59 File Downloads  -  Detailed Statistics Created: Thu, 14 Aug 2014, 15:35:24 EST by Jane Moschetti

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.