Double label autoradiography : an improvement

Wynford-Thomas, D., LaMontagne, A. and Prescott, David M. 1986, Double label autoradiography : an improvement, Journal of histochemistry and cytochemistry, vol. 34, no. 10, pp. 1335-1336, doi: 10.1177/34.10.3745911.

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Title Double label autoradiography : an improvement
Author(s) Wynford-Thomas, D.
LaMontagne, A.ORCID iD for LaMontagne, A.
Prescott, David M.
Journal name Journal of histochemistry and cytochemistry
Volume number 34
Issue number 10
Start page 1335
End page 1336
Total pages 2
Publisher Histochemical Society
Place of publication Seattle, Wash.
Publication date 1986
ISSN 1551-5044
Summary A convenient method for double-label autoradiography is described that uses an aqueous mountant, Gelutol (polyvinyl alcohol), which keeps the gelatin spacer in the final autoradiograph permanently swollen to a thickness of around 18 microns in contrast to its 5 microns thickness during exposure of the autoradiograph. This greatly improves optical discrimination between upper and lower layers without the loss of sensitivity or resolution that would result if a 18 microns spacer were used during exposure.
Language eng
DOI 10.1177/34.10.3745911
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C2.1 Other contribution to refereed journal
Copyright notice ©1986, Histochemical Society
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Document type: Journal Article
Collections: Faculty of Health
School of Health and Social Development
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