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Characterisation of lipase fatty acid selectivity using novel omega-3 pNP-acyl esters

Nalder, Tim D., Marshall, Susan, Pfeffer, Frederick B. and Barrow, Colin J. 2014, Characterisation of lipase fatty acid selectivity using novel omega-3 pNP-acyl esters, Journal of functional foods, vol. 6, no. 1, pp. 259-269, doi: 10.1016/j.jff.2013.10.015.

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Title Characterisation of lipase fatty acid selectivity using novel omega-3 pNP-acyl esters
Author(s) Nalder, Tim D.
Marshall, Susan
Pfeffer, Frederick B.ORCID iD for Pfeffer, Frederick B. orcid.org/0000-0002-5441-6437
Barrow, Colin J.ORCID iD for Barrow, Colin J. orcid.org/0000-0002-2153-7267
Journal name Journal of functional foods
Volume number 6
Issue number 1
Start page 259
End page 269
Total pages 11
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2014-01-01
ISSN 1756-4646
Keyword(s) p-Nitrophenyl ester
EPA
DHA
lipase
selectivity
steglich esterification
Summary  Lipases have applications for the industrial processing of lipids, including concentrating and/or modifying fish oil derived omega-3 fatty acids, widely used as nutritional supplement and functional food ingredients. A range of para-nitrophenol (pNP) acyl esters were synthesised as a means to rapidly screen lipases for fatty acid selectivity using spectrophotometric detection. The chosen esters were based primarily on the most abundant fatty acids present in anchovy and tuna oils. pNP derivatives of C16:1 n-7, C18:1 n-9 (OA), C18:2 n-6 (LA), C18:3 n-3 (ALA), C20:5 n-3 (EPA) and C22:6 n-3 (DHA) were synthesised. Storage stability of these pNP derivatives was shown to be at least 6 months and all pNP derivatives, including those of EPA and DHA, were shown to be stable throughout the conditions of the assay. We applied the new assay substrates for the determination of fatty acid selectivity of five widely utilised lipases. Results showed that the lipase from Candida rugosa was the most selective in terms of omega-3 specificity, preferentially hydrolysing all other medium– long chain substrates. Lipases from Rhizomucor miehei and Thermomyces lanuginosa also showed selectivity, with a significant preference for saturated fatty acids. Candida Antarctica lipase B and Aspergillus niger lipase were the least selective.
Language eng
DOI 10.1016/j.jff.2013.10.015
Field of Research 060107 Enzymes
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2014, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30067001

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Created: Tue, 14 Oct 2014, 12:42:35 EST by Tim Nalder

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