Metformin counters the insulin-induced suppression of fatty acid oxidation and stimulation of triacylglycerol storage in rodent skeletal muscle

Collier, Cheryl A., Bruce, Clinton R., Smith, Angela C., Lopaschuk, Gary and Dyck, David J. 2006, Metformin counters the insulin-induced suppression of fatty acid oxidation and stimulation of triacylglycerol storage in rodent skeletal muscle, American journal of physiology : endocrinology and metabolism, vol. 291, no. 1, pp. E182-E189, doi: 10.1152/ajpendo.00272.2005.

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Title Metformin counters the insulin-induced suppression of fatty acid oxidation and stimulation of triacylglycerol storage in rodent skeletal muscle
Author(s) Collier, Cheryl A.
Bruce, Clinton R.ORCID iD for Bruce, Clinton R. orcid.org/0000-0002-0515-3343
Smith, Angela C.
Lopaschuk, Gary
Dyck, David J.
Journal name American journal of physiology : endocrinology and metabolism
Volume number 291
Issue number 1
Start page E182
End page E189
Total pages 8
Publisher American Physiological Society
Place of publication Bethesada, Md.
Publication date 2006
ISSN 0193-1849
1522-1555
Keyword(s) Adenosine 5′-monophosphate-activated protein kinase
Diabetes
Lipid
Obesity
Summary The present study examined the acute effects of metformin on fatty acid (FA) metabolism in oxidative soleus (SOL) and glycolytic epitrochlearis (EPT) rodent muscle. SOL and EPT were incubated for either 30 or 180 min in the absence or presence of 2 mM metformin and with or without insulin (10 mU/ml). Metformin did not alter basal FA metabolism but countered the effects of insulin on FA oxidation and incorporation into triacylglyerol (TAG). Specifically, metformin prevented the insulin-induced suppression of FA oxidation in SOL but did not alter FA incorporation into lipid pools. In contrast, in EPT metformin blunted the incorporation of FA into TAG when insulin was present but did not alter FA oxidation. In SOL, metformin resulted in a 50% increase in AMP-activated protein kinase α2 activity and prevented the insulin-induced increase in malonyl-CoA content. In both fiber types, basal and insulin-stimulated glucose oxidation were not significantly altered by metformin. All effects were similar regardless of whether they were measured after 30 or 180 min. Because increased muscle lipid storage and impaired FA oxidation have been associated with insulin resistance in this tissue, the ability of metformin to reverse these abnormalities in muscle FA metabolism may be a part of the mechanism by which metformin improves glucose clearance and insulin sensitivity. The present data also suggest that increased glucose clearance is not due to its enhanced subsequent oxidation. Additional studies are warranted to determine whether chronic metformin treatment has similar effects on muscle FA metabolism.
Language eng
DOI 10.1152/ajpendo.00272.2005
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30067049

Document type: Journal Article
Collections: Faculty of Health
School of Health and Social Development
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