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Creatine transporter (SLC6A8) knockout mice display an increased capacity for in vitro creatine biosynthesis in skeletal muscle.

Russell,AP, Ghobrial,L, Wright,CR, Lamon,S, Brown,EL, Kon,M, Skelton,MR and Snow,RJ 2014, Creatine transporter (SLC6A8) knockout mice display an increased capacity for in vitro creatine biosynthesis in skeletal muscle., Frontiers in physiology, vol. 5, pp. 1-6, doi: 10.3389/fphys.2014.00314.

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Title Creatine transporter (SLC6A8) knockout mice display an increased capacity for in vitro creatine biosynthesis in skeletal muscle.
Author(s) Russell,APORCID iD for Russell,AP orcid.org/0000-0002-7323-9501
Ghobrial,L
Wright,CRORCID iD for Wright,CR orcid.org/0000-0001-7903-3144
Lamon,SORCID iD for Lamon,S orcid.org/0000-0002-3271-6551
Brown,EL
Kon,M
Skelton,MR
Snow,RJORCID iD for Snow,RJ orcid.org/0000-0002-4796-6916
Journal name Frontiers in physiology
Volume number 5
Start page 1
End page 6
Total pages 6
Publisher Frontiers
Place of publication Lausanne, Switzerland
Publication date 2014
ISSN 1664-042X
Keyword(s) creatine
energy metabolism
skeletal muscle
transgenic
Summary The present study aimed to investigate whether skeletal muscle from whole body creatine transporter (CrT; SLC6A8) knockout mice (CrT(-/y)) actually contained creatine (Cr) and if so, whether this Cr could result from an up regulation of muscle Cr biosynthesis. Gastrocnemius muscle from CrT(-/y) and wild type (CrT(+/y)) mice were analyzed for ATP, Cr, Cr phosphate (CrP), and total Cr (TCr) content. Muscle protein and gene expression of the enzymes responsible for Cr biosynthesis L-arginine:glycine amidotransferase (AGAT) and guanidinoacetate methyltransferase (GAMT) were also determined as were the rates of in vitro Cr biosynthesis. CrT(-/y) mice muscle contained measurable (22.3 ± 4.3 mmol.kg(-1) dry mass), but markedly reduced (P < 0.05) TCr levels compared with CrT(+/y) mice (125.0 ± 3.3 mmol.kg(-1) dry mass). AGAT gene and protein expression were higher (~3 fold; P < 0.05) in CrT(-/y) mice muscle, however GAMT gene and protein expression remained unchanged. The in vitro rate of Cr biosynthesis was elevated 1.5 fold (P < 0.05) in CrT(-/y) mice muscle. These data clearly demonstrate that in the absence of CrT protein, skeletal muscle has reduced, but not absent, levels of Cr. This presence of Cr may be at least partly due to an up regulation of muscle Cr biosynthesis as evidenced by an increased AGAT protein expression and in vitro Cr biosynthesis rates in CrT(-/y) mice. Of note, the up regulation of Cr biosynthesis in CrT(-/y) mice muscle was unable to fully restore Cr levels to that found in wild type muscle.
Language eng
DOI 10.3389/fphys.2014.00314
Field of Research 060699 Physiology not elsewhere classified
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Frontiers
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30067315

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.