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EPO-receptor is present in mouse C2C12 and human primary skeletal muscle cells but EPO does not influence myogenesis.

Lamon,S, Zacharewicz,E, Stephens,AN and Russell,AP 2014, EPO-receptor is present in mouse C2C12 and human primary skeletal muscle cells but EPO does not influence myogenesis., Physiological reports, vol. 2, no. 3, pp. 1-22, doi: 10.1002/phy2.256.

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Title EPO-receptor is present in mouse C2C12 and human primary skeletal muscle cells but EPO does not influence myogenesis.
Author(s) Lamon,SORCID iD for Lamon,S orcid.org/0000-0002-3271-6551
Zacharewicz,E
Stephens,AN
Russell,APORCID iD for Russell,AP orcid.org/0000-0002-7323-9501
Journal name Physiological reports
Volume number 2
Issue number 3
Start page 1
End page 22
Total pages 22
Publisher John Wiley & Sons
Place of publication Oxford, England
Publication date 2014
ISSN 2051-817X
Keyword(s) Cytokine
erythropoietin
erythropoietin‐receptor
myogenesis
skeletal muscle
Summary Abstract The role and regulation of the pleiotropic cytokine erythropoietin (EPO) in skeletal muscle are controversial. EPO exerts its effects by binding its specific receptor (EPO-R), which activates intracellular signaling and gene transcription in response to internal and external stress signals. EPO is suggested to play a direct role in myogenesis via the EPO-R, but several studies have questioned the effect of EPO treatment in muscle in vitro and in vivo. The lack of certainty surrounding the use of nonspecific EPO-R antibodies contributes to the ambiguity of the field. Our study demonstrates that the EPO-R gene and protein are expressed at each stage of mouse C2C12 and human skeletal muscle cell proliferation and differentiation and validates a specific antibody for the detection of the EPO-R protein. However, in our experimental conditions, EPO treatment had no effect on mouse C2C12 and human muscle cell proliferation, differentiation, protein synthesis or EPO-R expression. While an increase in Akt and MAPK phosphorylation was observed, we demonstrate that this effect resulted from the stress caused by changing medium and not from EPO treatment. We therefore suggest that skeletal muscle EPO-R might be present in a nonfunctional form, or too lowly expressed to play a role in muscle cell function.
Language eng
DOI 10.1002/phy2.256
Field of Research 060699 Physiology not elsewhere classified
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Wiley
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30067316

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.