A strain-promoted alkyne-azide cycloaddition (SPAAC) reaction of a novel EpCAM aptamer-fluorescent conjugate for imaging of cancer cells

Subramanian, N, Sreemanthula, JB, Balaji, B, Kanwar, JR, Biswas, J and Krishnakumar, S 2014, A strain-promoted alkyne-azide cycloaddition (SPAAC) reaction of a novel EpCAM aptamer-fluorescent conjugate for imaging of cancer cells, Chemical Communications, vol. 50, no. 80, pp. 11810-11813, doi: 10.1039/c4cc02996h.

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Title A strain-promoted alkyne-azide cycloaddition (SPAAC) reaction of a novel EpCAM aptamer-fluorescent conjugate for imaging of cancer cells
Author(s) Subramanian, N
Sreemanthula, JB
Balaji, B
Kanwar, JRORCID iD for Kanwar, JR orcid.org/0000-0003-3728-9568
Biswas, J
Krishnakumar, S
Journal name Chemical Communications
Volume number 50
Issue number 80
Start page 11810
End page 11813
Total pages 4
Publisher Royal Society of Chemistry
Place of publication Cambridge, England
Publication date 2014-10-14
ISSN 1364-548X
Keyword(s) Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Chemistry
TARGETED DRUG-DELIVERY
ADHESION MOLECULE APTAMER
CLICK CONJUGATION
SELECTION
THERAPY
Summary For the first time, a novel EpCAM aptamer (SYL3C)-DIBO-AF594 fluorescent conjugate was synthesised by bioorthogonal chemistry utilizing a strain promoted alkyne-azide cycloaddition (copper free click) reaction (SPAAC). The ligation efficiency of SPAAC was improved by freeze-thaw cycles. The obtained conjugate showed target specific binding and aided in the imaging of various EpCAM positive cancer cell lines like MCF7, MDAMB453, Weri-RB1 and PC3.
Language eng
DOI 10.1039/c4cc02996h
Field of Research 111201 Cancer Cell Biology
Socio Economic Objective 920102 Cancer and Related Disorders
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Royal Society of Chemistry
Persistent URL http://hdl.handle.net/10536/DRO/DU:30067541

Document type: Journal Article
Collection: School of Medicine
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