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Evaluation of the genotoxic potential against H2O2-radical-mediated DNA damage and acute oral toxicity of standardized extract of Polyalthia longifolia leaf

Jothy, Subramanion L., Chen, Yeng, Kanwar, Jagat R. and Sasidharan, Sreenivasan 2013, Evaluation of the genotoxic potential against H2O2-radical-mediated DNA damage and acute oral toxicity of standardized extract of Polyalthia longifolia leaf, Evidence-based complementary and alternative medicine, pp. 1-13, doi: 10.1155/2013/925380.

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Title Evaluation of the genotoxic potential against H2O2-radical-mediated DNA damage and acute oral toxicity of standardized extract of Polyalthia longifolia leaf
Author(s) Jothy, Subramanion L.
Chen, Yeng
Kanwar, Jagat R.ORCID iD for Kanwar, Jagat R. orcid.org/0000-0003-3728-9568
Sasidharan, Sreenivasan
Journal name Evidence-based complementary and alternative medicine
Article ID 925380
Start page 1
End page 13
Total pages 13
Publisher Hindawi Publishing Corporation
Place of publication Cairo, Egypt
Publication date 2013
ISSN 1741-427X
Keyword(s) Science & Technology
Life Sciences & Biomedicine
Integrative & Complementary Medicine
Summary Medicinal plants have been used in medicoculturally diverse countries around the world, where it is a part of a time-honoured tradition that is respected even today. Polyalthia longifolia leaf extract has been previously reported as an efficient antioxidant in vitro. Hence, the genotoxic effects of P. longifolia leaf were investigated by using plasmid relation, comet, and Allium cepa assay. In the presence of  OH radicals, the DNA in supercoil was start nicked into open circular form, which is the product of the single-stranded cleavage of supercoil DNA and quantified as fragmented separate bands on agarose gel in plasmid relation assay. In the plasmid relation and comet assay, the P. longifolia leaf extract exhibited strong inhibitory effects against H2O2-mediated DNA damage. A dose-dependent increase of chromosome aberrations was also observed in the Allium cepa assay. The abnormalities scored were stickiness, c-mitosis, bridges, and vagrant chromosomes. Micronucleated cells were also observed at the interphase. The results of Allium cepa assay confirmed that the methanol extracts of P. longifolia exerted no significant genotoxic or mitodepressive effects at 100 μg/mL. Thus, this study demonstrated that P. longifolia leaf extract has a beneficial effect against oxidative DNA damage. This experiment is the first report for the protective effect of P. longifolia on DNA damage-induced by hydroxyl radicals. Additionally in acute oral toxicity study, female rats were treated at 5000 mg/kg body weight of P. longifolia leaf extract and observed for signs of toxicity for 14 days. P. longifolia leaf extract did not produce any treatment-related toxic effects in rats.
Language eng
DOI 10.1155/2013/925380
Field of Research 111299 Oncology and Carcinogenesis not elsewhere classified
1104 Complementary And Alternative Medicine
Socio Economic Objective 920102 Cancer and Related Disorders
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2013, Subramanion L. Jothy et al.
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30067575

Document type: Journal Article
Collections: School of Medicine
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.