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FTIR microspectroscopy for rapid screening and monitoring of polyunsaturated fatty acid production in commercially valuable marine yeasts and protists

Vongsvivut, Jitraporn, Heraud, Philip, Gupta, Adarsha, Puri, Munish, McNaughton, Don and Barrow, Colin J. 2013, FTIR microspectroscopy for rapid screening and monitoring of polyunsaturated fatty acid production in commercially valuable marine yeasts and protists, Analyst, vol. 138, no. 20, pp. 6016-6031, doi: 10.1039/c3an00485f.

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Title FTIR microspectroscopy for rapid screening and monitoring of polyunsaturated fatty acid production in commercially valuable marine yeasts and protists
Author(s) Vongsvivut, Jitraporn
Heraud, Philip
Gupta, Adarsha
Puri, Munish
McNaughton, Don
Barrow, Colin J.
Journal name Analyst
Volume number 138
Issue number 20
Start page 6016
End page 6031
Total pages 16
Publisher Royal Society of Chemistry Publications
Place of publication London, Eng.
Publication date 2013
ISSN 1364-5528
Keyword(s) Science & Technology
Physical Sciences
Chemistry, Analytical
Chemistry
TRANSFORM INFRARED-SPECTROSCOPY
IR SPECTROSCOPY
IDENTIFICATION
SPECTRA
TISSUE
CELLS
BANDS
CLASSIFICATION
PEROXIDATION
MEMBRANES
Summary The increase in polyunsaturated fatty acid (PUFA) consumption has prompted research into alternative resources other than fish oil. In this study, a new approach based on focal-plane-array Fourier transform infrared (FPA-FTIR) microspectroscopy and multivariate data analysis was developed for the characterisation of some marine microorganisms. Cell and lipid compositions in lipid-rich marine yeasts collected from the Australian coast were characterised in comparison to a commercially available PUFA-producing marine fungoid protist, thraustochytrid. Multivariate classification methods provided good discriminative accuracy evidenced from (i) separation of the yeasts from thraustochytrids and distinct spectral clusters among the yeasts that conformed well to their biological identities, and (ii) correct classification of yeasts from a totally independent set using cross-validation testing. The findings further indicated additional capability of the developed FPA-FTIR methodology, when combined with partial least squares regression (PLSR) analysis, for rapid monitoring of lipid production in one of the yeasts during the growth period, which was achieved at a high accuracy compared to the results obtained from the traditional lipid analysis based on gas chromatography. The developed FTIR-based approach when coupled to programmable withdrawal devices and a cytocentrifugation module would have strong potential as a novel online monitoring technology suited for bioprocessing applications and large-scale production.
Language eng
DOI 10.1039/c3an00485f
Field of Research 030606 Structural Chemistry and Spectroscopy
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2013, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30067751

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.