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Challenges and advances in the heterologous expression of cellulolytic enzymes: a review

Lambertz, Camilla, Garvey, Megan, Klinger, Johannes, Heesel, Dirk, Klose, Holger, Fischer, Rainer and Commandeur, Ulrich 2014, Challenges and advances in the heterologous expression of cellulolytic enzymes: a review, Biotechnology for biofuels, vol. 7, no. 1, pp. 1-15, doi: 10.1186/s13068-014-0135-5.

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Title Challenges and advances in the heterologous expression of cellulolytic enzymes: a review
Author(s) Lambertz, Camilla
Garvey, Megan
Klinger, Johannes
Heesel, Dirk
Klose, Holger
Fischer, Rainer
Commandeur, Ulrich
Journal name Biotechnology for biofuels
Volume number 7
Issue number 1
Start page 1
End page 15
Total pages 15
Publisher BioMed Central
Place of publication London, England
Publication date 2014
ISSN 1754-6834
Keyword(s) Cellulase
Cellulosome
Consolidated bioprocessing
Heterologous expression
Industrial processing
Summary Second generation biofuel development is increasingly reliant on the recombinant expression of cellulases. Designing or identifying successful expression systems is thus of preeminent importance to industrial progress in the field. Recombinant production of cellulases has been performed using a wide range of expression systems in bacteria, yeasts and plants. In a number of these systems, particularly when using bacteria and plants, significant challenges have been experienced in expressing full-length proteins or proteins at high yield. Further difficulties have been encountered in designing recombinant systems for surface-display of cellulases and for use in consolidated bioprocessing in bacteria and yeast. For establishing cellulase expression in plants, various strategies are utilized to overcome problems, such as the auto-hydrolysis of developing plant cell walls. In this review, we investigate the major challenges, as well as the major advances made to date in the recombinant expression of cellulases across the commonly used bacterial, plant and yeast systems. We review some of the critical aspects to be considered for industrial-scale cellulase production.
Language eng
DOI 10.1186/s13068-014-0135-5
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30068185

Document type: Journal Article
Collections: School of Medicine
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.