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Specific recognition of the HIV-1 genomic RNA by the Gag precursor

Abd El-Wahab,EW, Smyth,RP, Mailler,E, Bernacchi,S, Vivet-Boudou,V, Hijnen,M, Jossinet,F, Mak,J, Paillart,JC and Marquet,R 2014, Specific recognition of the HIV-1 genomic RNA by the Gag precursor, Nature communications, vol. 5, pp. 1-13, doi: 10.1038/ncomms5304.

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Title Specific recognition of the HIV-1 genomic RNA by the Gag precursor
Author(s) Abd El-Wahab,EW
Smyth,RP
Mailler,E
Bernacchi,S
Vivet-Boudou,V
Hijnen,M
Jossinet,F
Mak,J
Paillart,JC
Marquet,R
Journal name Nature communications
Volume number 5
Start page 1
End page 13
Total pages 13
Publisher Macmillan Publishers
Place of publication London, England
Publication date 2014
ISSN 2041-1723
Keyword(s) Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
HUMAN-IMMUNODEFICIENCY-VIRUS
SPLICED VIRAL-RNA
TYPE-1 RNA
NUCLEOCAPSID PROTEIN
PACKAGING SIGNALS
REVERSE TRANSCRIPTION
MUTATIONAL ANALYSIS
DIMER LINKAGE
DIMERIZATION
ENCAPSIDATION
Summary During assembly of HIV-1 particles in infected cells, the viral Pr55(Gag) protein (or Gag precursor) must select the viral genomic RNA (gRNA) from a variety of cellular and viral spliced RNAs. However, there is no consensus on how Pr55(Gag) achieves this selection. Here, by using RNA binding and footprinting assays, we demonstrate that the primary Pr55(Gag) binding site on the gRNA consists of the internal loop and the lower part of stem-loop 1 (SL1), the upper part of which initiates gRNA dimerization. A double regulation ensures specific binding of Pr55(Gag) to the gRNA despite the fact that SL1 is also present in spliced viral RNAs. The region upstream of SL1, which is present in all HIV-1 RNAs, prevents binding to SL1, but this negative effect is counteracted by sequences downstream of SL4, which are unique to the gRNA.
Language eng
DOI 10.1038/ncomms5304
Field of Research 110106 Medical Biochemistry: Proteins and Peptides (incl Medical Proteomics)
110804 Medical Virology
Socio Economic Objective 920109 Infectious Diseases
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Grant ID NHMRC 543107
Copyright notice ©2014, Macmillan Publishers Limited
Persistent URL http://hdl.handle.net/10536/DRO/DU:30069005

Document type: Journal Article
Collection: School of Medicine
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