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Validation of a multiplex reverse transcription and pre-amplification method using TaqMan(®) MicroRNA assays.

Le Carré,J, Lamon,S and Léger,B 2014, Validation of a multiplex reverse transcription and pre-amplification method using TaqMan(®) MicroRNA assays., Frontiers in genetics, vol. 5, pp. 413-1, doi: 10.3389/fgene.2014.00413.

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Title Validation of a multiplex reverse transcription and pre-amplification method using TaqMan(®) MicroRNA assays.
Author(s) Le Carré,J
Lamon,SORCID iD for Lamon,S orcid.org/0000-0002-3271-6551
Léger,B
Journal name Frontiers in genetics
Volume number 5
Start page 413
End page 1
Total pages 7
Publisher Frontiers Research Foundation
Place of publication Lausanne, Switzerland
Publication date 2014-11-26
ISSN 1664-8021
Keyword(s) human skeletal muscle
miRNA
multiplexing
plasma
qRT-PCR
Summary Since the discovery of microRNAs (miRNAs), different approaches have been developed to label, amplify and quantify miRNAs. The TaqMan(®) technology, provided by Applied Biosystems (ABIs), uses a stem-loop reverse transcription primer system to reverse transcribe the RNA and amplify the cDNA. This method is widely used to identify global differences between the expression of 100s of miRNAs across comparative samples. This technique also allows the quantification of the expression of targeted miRNAs to validate observations determined by whole-genome screening or to analyze few specific miRNAs on a large number of samples. Here, we describe the validation of a method published by ABIs on their web site allowing to reverse transcribe and pre-amplify multiple miRNAs and snoRNAs simultaneously. The validation of this protocol was performed on human muscle and plasma samples. Fast and cost efficient, this method achieves an easy and convenient way to screen a relatively large number of miRNAs in parallel.
Language eng
DOI 10.3389/fgene.2014.00413
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2014, Frontiers Research Foundation
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30069042

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.