Synchrotron-FTIR microspectroscopy enables the distinction of lipid accumulation in thraustochytrid strains through analysis of individual live cells

Vongsvivut,J, Heraud,P, Gupta,A, Thyagarajan,T, Puri,M, McNaughton,D and Barrow,CJ 2015, Synchrotron-FTIR microspectroscopy enables the distinction of lipid accumulation in thraustochytrid strains through analysis of individual live cells, Protist, vol. 166, no. 1, pp. 106-121, doi: 10.1016/j.protis.2014.12.002.

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Title Synchrotron-FTIR microspectroscopy enables the distinction of lipid accumulation in thraustochytrid strains through analysis of individual live cells
Author(s) Vongsvivut,J
Heraud,P
Gupta,A
Thyagarajan,T
Puri,MORCID iD for Puri,M orcid.org/0000-0003-2469-3326
McNaughton,D
Barrow,CJORCID iD for Barrow,CJ orcid.org/0000-0002-2153-7267
Journal name Protist
Volume number 166
Issue number 1
Start page 106
End page 121
Publisher Elsevier GmbH
Place of publication Muenchen, Germany
Publication date 2015-02
ISSN 1434-4610
1618-0941
Keyword(s) Thraustochytrids
lipids
multivariate data analysis.
polyunsaturated fatty acids
synchrotron Fourier transform infrared (S-FTIR) microspectroscopy
Summary The superior characteristics of high photon flux and diffraction-limited spatial resolution achieved by synchrotron-FTIR microspectroscopy allowed molecular characterization of individual live thraustochytrids. Principal component analysis revealed distinct separation of the single live cell spectra into their corresponding strains, comprised of new Australasian thraustochytrids (AMCQS5-5 and S7) and standard cultures (AH-2 and S31). Unsupervised hierarchical cluster analysis (UHCA) indicated close similarities between S7 and AH-7 strains, with AMCQS5-5 being distinctly different. UHCA correlation conformed well to the fatty acid profiles, indicating the type of fatty acids as a critical factor in chemotaxonomic discrimination of these thraustochytrids and also revealing the distinctively high polyunsaturated fatty acid content as key identity of AMCQS5-5. Partial least squares discriminant analysis using cross-validation approach between two replicate datasets was demonstrated to be a powerful classification method leading to models of high robustness and 100% predictive accuracy for strain identification. The results emphasized the exceptional S-FTIR capability to perform real-time in vivo measurement of single live cells directly within their original medium, providing unique information on cell variability among the population of each isolate and evidence of spontaneous lipid peroxidation that could lead to deeper understanding of lipid production and oxidation in thraustochytrids for single-cell oil development.
Language eng
DOI 10.1016/j.protis.2014.12.002
Field of Research 100302 Bioprocessing, Bioproduction and Bioproducts
Socio Economic Objective 920411 Nutrition
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Elsevier GmbH
Persistent URL http://hdl.handle.net/10536/DRO/DU:30069150

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