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In vitro and in situ screening systems for morphological and phytochemical analysis of Withania somnifera germplasms

Johny,L, Conlan,X, Cahill,D and Adholeya,A 2014, In vitro and in situ screening systems for morphological and phytochemical analysis of Withania somnifera germplasms, Plant cell, tissue and organ culture, vol. 120, no. 3, pp. 1-12, doi: 10.1007/s11240-014-0673-3.

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Title In vitro and in situ screening systems for morphological and phytochemical analysis of Withania somnifera germplasms
Author(s) Johny,L
Conlan,XORCID iD for Conlan,X orcid.org/0000-0003-0829-0551
Cahill,DORCID iD for Cahill,D orcid.org/0000-0002-2556-0528
Adholeya,A
Journal name Plant cell, tissue and organ culture
Volume number 120
Issue number 3
Start page 1
End page 12
Total pages 12
Publisher Springer
Place of publication Dordrecht , Netherlands
Publication date 2014-12-13
ISSN 0167-6857
Keyword(s) HPLC
In situ
In vitro
Withaferin A
Withania somnifera
Withanolides
Summary We report, for the first time for Withania somnifera, the use of a modified in vitro system for morphological and phytochemical screening of true to type plants as compared with those grown in a conventional in situ system. Eleven germplasms of cultivated W. somnifera from different regions of India were collected to examine chemotypic variation in withaferin A (WA). Methods were developed to optimize WA extraction. The maximum concentration of WA was extracted from manually ground leaf and root material to which 60 % methanol was added followed by sonication in a water bath sonicator. Variation in WA concentration in whole plants was observed amongst the different germplasms. In the in vitro system, the concentration of WA ranged between 0.27 and 7.64 mg/g dry weight (DW) and in the in situ system, the range in concentration was between 8.06 and 36.31 mg/g DW. The highest amount of WA found in leaves was 7.37 and 41.42 mg/g DW in the in vitro and the in situ systems respectively. In roots, the highest WA concentration was 0.27 mg/g DW in the in vitro and 0.60 mg/g DW in the in situ system. There are distinct advantages in using the in vitro grown plants rather than those grown in the in situ system including the simplicity of design, efficient use of space and nutrition and a system which is soil and contaminant free. The proposed in vitro system is therefore ideal for utilization in molecular, enzymatic and biochemical studies.
Language eng
DOI 10.1007/s11240-014-0673-3
Field of Research 060705 Plant Physiology
Socio Economic Objective 820209 Ornamentals
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30069157

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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