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Enhancing permanganate chemiluminescence detection for the determination of glutathione and glutathione disulfide in biological matrices

Smith, Zoe M., Terry, Jessica M., Barnett, Neil W., Gray, Laura J., Wright, Dean J. and Francis, Paul S. 2014, Enhancing permanganate chemiluminescence detection for the determination of glutathione and glutathione disulfide in biological matrices, Analyst, vol. 139, no. 10, pp. 2416-2422, doi: 10.1039/c4an00255e.

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Title Enhancing permanganate chemiluminescence detection for the determination of glutathione and glutathione disulfide in biological matrices
Author(s) Smith, Zoe M.
Terry, Jessica M.
Barnett, Neil W.
Gray, Laura J.ORCID iD for Gray, Laura J. orcid.org/0000-0002-7903-5796
Wright, Dean J.
Francis, Paul S.ORCID iD for Francis, Paul S. orcid.org/0000-0003-4165-6922
Journal name Analyst
Volume number 139
Issue number 10
Start page 2416
End page 2422
Total pages 7
Publisher Royal Society of Chemistry
Place of publication London, Eng.
Publication date 2014
ISSN 1364-5528
Keyword(s) Science & Technology
Physical Sciences
Chemistry, Analytical
Chemistry
ACIDIC POTASSIUM-PERMANGANATE
PERFORMANCE LIQUID-CHROMATOGRAPHY
HUNTINGTONS-DISEASE
OXIDATIVE STRESS
THIOLS
MANGANESE(II)
REAGENT
Summary Acidic potassium permanganate chemiluminescence enables direct post-column detection of glutathione, but its application to assess the redox state of a wider range of biological fluids and tissues is limited by its sensitivity. Herein we show that the simple on-line addition of an aqueous formaldehyde solution not only enhances the sensitivity of the procedure by two orders of magnitude, but also provides a remarkable improvement in the selectivity of the reagent towards thiols such as glutathione (compared to phenols and amino acids that do not possess a thiol group). This enhanced mode of detection was applied to the determination of glutathione and its corresponding disulfide species in homogenised striatum samples taken from both wild type mice and the R6/1 transgenic mouse model of Huntington's disease, at both 8 and 12 weeks of age. No significant difference was observed between the GSH/GSSG ratios of wild type mice and R6/1 mice at either age group, suggesting that the early disease progression had not significantly altered the intracellular redox environment.
Language eng
DOI 10.1039/c4an00255e
Field of Research 030108 Separation Science
030101 Analytical Spectrometry
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Grant ID FT100100646
FT100100835
Copyright notice ©2014, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30069420

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.