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A novel in vitro whole plant system for analysis of polyphenolics and their antioxidant potential in cultivars of Ocimum basilicum

Srivastava, Shivani, Cahill, David M., Conlan, Xavier A. and Adholeya, Alok 2014, A novel in vitro whole plant system for analysis of polyphenolics and their antioxidant potential in cultivars of Ocimum basilicum, Journal of agricultural and food chemistry, vol. 62, no. 41, pp. 10064-10075, doi: 10.1021/jf502709e.

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Title A novel in vitro whole plant system for analysis of polyphenolics and their antioxidant potential in cultivars of Ocimum basilicum
Author(s) Srivastava, Shivani
Cahill, David M.ORCID iD for Cahill, David M. orcid.org/0000-0002-2556-0528
Conlan, Xavier A.ORCID iD for Conlan, Xavier A. orcid.org/0000-0003-0829-0551
Adholeya, Alok
Journal name Journal of agricultural and food chemistry
Volume number 62
Issue number 41
Start page 10064
End page 10075
Total pages 12
Publisher ACS Publications
Place of publication Washington, D.C.
Publication date 2014
ISSN 1520-5118
Keyword(s) Ocimum basilicum
antioxidant
basil
bioprospecting
chemiluminescence
chicoric acid
rosmarinic acid
Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Agriculture, Multidisciplinary
Chemistry, Applied
Food Science & Technology
Agriculture
Chemistry
ACIDIC POTASSIUM-PERMANGANATE
CHEMICAL-COMPOSITION
PHENOLIC COMPOSITION
L. CULTIVARS
SWEET BASIL
CULTURES
LEAVES
ASSAYS
Summary Plants are an important source for medicinal compounds. Chemical screening and selection is critical for identification of compounds of interest. Ocimum basilicum (Basil) is a rich source of polyphenolics and exhibits high diversity, therefore bioprospecting of a suitable cultivar is a necessity. This study reports on the development of a true to type novel "in vitro system" and its comparison with a conventional system for screening and selection of cultivars for high total phenolics, individual polyphenolics, and antioxidant content. We have shown for the first time using online acidic potassium permanganate chemiluminescence that extracts from Ocimum basilicum showed antioxidant potential. The current study identified the cultivar specific composition of polyphenolics and their antioxidant properties. Further, a distinct relationship between plant morphotype and polyphenolic content was also found. Of the 15 cultivars examined, "Holy Green", "Red Rubin", and "Basil Genovese" were identified as high polyphenolic producing cultivars while "Subja" was determined to be a low producer. The "in vitro system" enabled differentiation of the cultivars in their morphology, polyphenolic content, and antioxidant activity and is a cheap and efficient method for bioprospecting studies.
Language eng
DOI 10.1021/jf502709e
Field of Research 060703 Plant Developmental and Reproductive Biology
060705 Plant Physiology
030108 Separation Science
Socio Economic Objective 820303 Plant Extract Crops (e.g. Pyrethrum, Jojoba)
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, ACS Publications
Free to Read? Yes
Use Rights Creative Commons Attribution noncommercial no derivatives licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30069817

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.