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Fatty acid-specific alterations in leptin, PPARα, and CPT-1 gene expression in the rainbow trout

Coccia, Elena, Varricchio, Ettore, Vito, Pasquale, Turchini, Giovanni M., Francis, David Scott and Paolucci, Marina 2014, Fatty acid-specific alterations in leptin, PPARα, and CPT-1 gene expression in the rainbow trout, Lipids, vol. 49, no. 10, pp. 1033-1046, doi: 10.1007/s11745-014-3939-y.

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Title Fatty acid-specific alterations in leptin, PPARα, and CPT-1 gene expression in the rainbow trout
Author(s) Coccia, Elena
Varricchio, Ettore
Vito, Pasquale
Turchini, Giovanni M.ORCID iD for Turchini, Giovanni M. orcid.org/0000-0003-0694-4283
Francis, David ScottORCID iD for Francis, David Scott orcid.org/0000-0002-4829-6926
Paolucci, Marina
Journal name Lipids
Volume number 49
Issue number 10
Start page 1033
End page 1046
Total pages 14
Publisher Springer
Place of publication Heidelberg , Germany
Publication date 2014-08-10
ISSN 1558-9307
Keyword(s) CPT-1
Fatty acids
Leptin
Liver
PPARα
Rainbow trout
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Nutrition & Dietetics
PPAR alpha
SALMON SALMO-SALAR
FISH-OIL REPLACEMENT
PROLIFERATOR-ACTIVATED RECEPTORS
CONJUGATED LINOLEIC-ACID
ONCORHYNCHUS-MYKISS
LIPID-METABOLISM
DOCOSAHEXAENOIC ACID
VEGETABLE-OIL
LONG-CHAIN
GENOME DUPLICATION
Summary It is known that fatty acids (FA) regulate lipid metabolism by modulating the expression of numerous genes. In order to gain a better understanding of the effect of individual FA on lipid metabolism related genes in rainbow trout (Oncorhynchus mykiss), an in vitro time-course study was implemented where twelve individual FA (butyric 4:0; caprylic 8:0; palmitic (PAM) 16:0; stearic (STA) 18:0; palmitoleic16:1n-7; oleic 18:1n-9; 11-cis-eicosenoic 20:1n-9; linoleic (LNA) 18:2n-6; α-linolenic (ALA) 18:3n-3; eicosapentenoic (EPA) 20:5n-3; docosahexaenoic (DHA) 22:6n-3; arachidonic (ARA) 20:4n-6) were incubated in rainbow trout liver slices. The effect of FA administration over time was evaluated on the expression of leptin, PPARα and CPT-1 (lipid oxidative related genes). Leptin mRNA expression was down regulated by saturated fatty acids (SFA) and LNA, and was up regulated by monounsaturated fatty acids (MUFA) and long chain PUFA, whilst STA and ALA had no effect. PPARα and CPT-1mRNA expression were up regulated by SFA, MUFA, ALA, ARA and DHA; and down regulated by LNA and EPA. These results suggest that there are individual and specific FA induced modifications of leptin, PPARα and CPT-1 gene expression in rainbow trout, and it is envisaged that such results may provide highly valuable information for future practical applications in fish nutrition.
Language eng
DOI 10.1007/s11745-014-3939-y
Field of Research 070401 Aquaculture
Socio Economic Objective 830102 Aquaculture Fin Fish (excl. Tuna)
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30069905

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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