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Real-time measurement of phloem turgor pressure in Hevea brasiliensis with a modified cell pressure probe

An, Feng, Cahill, David, Rookes, James, Lin, Weifu and Kong, Lingxue 2014, Real-time measurement of phloem turgor pressure in Hevea brasiliensis with a modified cell pressure probe, Botanical Studies, vol. 55, no. 1, pp. 1-11, doi: 10.1186/1999-3110-55-19.

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Title Real-time measurement of phloem turgor pressure in Hevea brasiliensis with a modified cell pressure probe
Author(s) An, Feng
Cahill, DavidORCID iD for Cahill, David orcid.org/0000-0002-2556-0528
Rookes, JamesORCID iD for Rookes, James orcid.org/0000-0003-1479-2339
Lin, Weifu
Kong, LingxueORCID iD for Kong, Lingxue orcid.org/0000-0001-6219-3897
Journal name Botanical Studies
Volume number 55
Issue number 1
Start page 1
End page 11
Total pages 11
Publisher SpringerOpen
Place of publication London, Eng.
Publication date 2014-02-03
ISSN 1817-406X
Keyword(s) Cell pressure probe
Hevea brasiliensis
Phloem turgor pressure
Phloem water relationship
Pressure flow theory
Sap flow
Tapping
Science & Technology
Life Sciences & Biomedicine
Plant Sciences
MUNCH HYPOTHESIS
SIEVE TUBES
LATICIFERS
GRADIENTS
PLANTS
Summary Background: Although the pressure flow theory is widely accepted for the transport of photoassimilates in phloem sieve elements, it still requires strong experimental validation. One reason for that is the lack of a precise method for measuring the real-time phloem turgor pressure from the sink tissues, especially in tree trunks.

Results: Taking the merits of Hevea brasiliensis, a novel phloem turgor pressure probe based on the state of the art cell pressure probe was developed. Our field measurements showed that the phloem turgor pressure probe can sensitively measure the real-time variation of phloem turgor pressure in H. brasiliensis but the calculation of phloem turgor pressure with xylem tension, xylem sap osmotic potential and phloem sap osmotic potential will under-estimate it. The measured phloem turgor pressure gradient in H. brasiliensis is contrary to the Münch theory. The phloem turgor pressure of H. brasiliensis varied from 8-12 bar as a consequence of water withdrawal from transpiration. Tapping could result in a sharp decrease of phloem turgor pressure followed by a recovery from 8-45 min after the tapping. The recovery of phloem turgor pressure after tapping and its change with xylem sap flow suggest the importance of phloem water relationship in the phloem turgor pressure regulation.

Conclusion: The phloem turgor pressure probe is a reliable technique for measuring the real-time variation of phloem turgor pressures in H. brasiliensis. The technique could probably be extended to the accurate measurement of phloem turgor pressure in other woody plants which is essential to test the Münch theory and to investigate the phloem water relationship and turgor pressure regulation.
Language eng
DOI 10.1186/1999-3110-55-19
Field of Research 060705 Plant Physiology
060799 Plant Biology not elsewhere classified
100101 Agricultural Biotechnology Diagnostics (incl Biosensors)
Socio Economic Objective 820102 Harvesting and Transport of Forest Products
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30070362

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.