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DryLab® optimised two-dimensional high performance liquid chromatography for differentiation of ephedrine and pseudoephedrine based methamphetamine samples

Andrighetto, Luke M., Stevenson, Paul G., Pearson, James R., Henderson, Luke C. and Conlan, Xavier A. 2014, DryLab® optimised two-dimensional high performance liquid chromatography for differentiation of ephedrine and pseudoephedrine based methamphetamine samples, Forensic science international, vol. 244, pp. 302-305, doi: 10.1016/j.forsciint.2014.09.018.

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Title DryLab® optimised two-dimensional high performance liquid chromatography for differentiation of ephedrine and pseudoephedrine based methamphetamine samples
Author(s) Andrighetto, Luke M.
Stevenson, Paul G.ORCID iD for Stevenson, Paul G. orcid.org/0000-0001-6780-6859
Pearson, James R.
Henderson, Luke C.ORCID iD for Henderson, Luke C. orcid.org/0000-0002-4244-2056
Conlan, Xavier A.ORCID iD for Conlan, Xavier A. orcid.org/0000-0003-0829-0551
Journal name Forensic science international
Volume number 244
Start page 302
End page 305
Publisher Elsevier Ireland
Place of publication Shannon, Ireland
Publication date 2014-11
ISSN 1872-6283
0379-0738
Keyword(s) Drylab®
Ephedrine
Methamphetamine
Multidimensional high performance liquid chromatography
Pseudoephedrine
Science & Technology
Life Sciences & Biomedicine
Medicine, Legal
Legal Medicine
Drylab (R)
SPECTROMETRY
SEPARATION
HPLC
Summary In-silico optimised two-dimensional high performance liquid chromatographic (2D-HPLC) separations of a model methamphetamine seizure sample are described, where an excellent match between simulated and real separations was observed. Targeted separation of model compounds was completed with significantly reduced method development time. This separation was completed in the heart-cutting mode of 2D-HPLC where C18 columns were used in both dimensions taking advantage of the selectivity difference of methanol and acetonitrile as the mobile phases. This method development protocol is most significant when optimising the separation of chemically similar chemical compounds as it eliminates potentially hours of trial and error injections to identify the optimised experimental conditions. After only four screening injections the gradient profile for both 2D-HPLC dimensions could be optimised via simulations, ensuring the baseline resolution of diastereomers (ephedrine and pseudoephedrine) in 9.7 min. Depending on which diastereomer is present the potential synthetic pathway can be categorized.
Language eng
DOI 10.1016/j.forsciint.2014.09.018
Field of Research 030108 Separation Science
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Elsevier Ireland
Persistent URL http://hdl.handle.net/10536/DRO/DU:30070493

Document type: Journal Article
Collection: Institute for Frontier Materials
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